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dc.creatorVilipić, Jovana P.
dc.creatorNovaković, Irena
dc.creatorZlatović, Mario
dc.creatorVujčić, Miroslava
dc.creatorTufegdžić, Srđan
dc.creatorSladić, Dušan
dc.date.accessioned2019-01-30T17:52:25Z
dc.date.available2019-01-30T17:52:25Z
dc.date.issued2016
dc.identifier.issn0352-5139
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2049
dc.description.abstractThe interactions of nine amino acid derivatives of tert-butylquinone with biomacromolecules were studied. Sodium dodecyl sulphate (SDS) gel electrophoresis and mass spectrometry confirmed the absence of modifications of lysozyme by any of the synthesized compounds. Spectrophotometric studies demonstrated hyperchromism, i.e., the existence of interactions between the quinones and calf thymus DNA (CT-DNA). Determination of the binding constants by absorption titration indicated weak interactions between the quinone derivatives and CT-DNA. The quenching of fluorescence of the intercalator ethidium bromide (EB) from the EB-CT-DNA system and of the minor groove binder Hoechst 33258 (H) from the H-CT-DNA system by the synthesized derivatives indicated interactions of the compounds and CT-DNA. Circular dichroism (CD) spectra demonstrated a non-intercalative binding mode of the quinone derivatives to CT-DNA. Molecular docking results confirmed binding to the minor groove. The electrophoretic pattern showed no cleavage of the pUC19 plasmid in the presence of any of the synthesized compounds. The ability of the derivatives to scavenge radicals was confirmed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. All the presented results suggest that the DNA minor groove binding is the principal mechanism of action of the examined amino acid derivatives.en
dc.publisherSerbian Chemical Soc, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172055/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/256716/EU//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectquinoneen
dc.subjectDNA bindingen
dc.subjectminor grooveen
dc.subjectfluorescenceen
dc.titleInteractions of cytotoxic amino acid derivatives of tert-butylquinone with DNA and lysozymeen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractСладиц, Дусан М.; Туфегджић, Срђан; Новаковић, Ирена; Вилипиц, Јована П.; Вујчић, Мирослава; Златовиц, Марио В.;
dc.citation.volume81
dc.citation.issue12
dc.citation.spage1345
dc.citation.epage1358
dc.citation.other81(12): 1345-1358
dc.citation.rankM23
dc.identifier.doi10.2298/JSC160725101V
dc.identifier.rcubConv_3669
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/8477/2047.pdf
dc.identifier.scopus2-s2.0-85009807714
dc.identifier.wos000392946100002
dc.type.versionpublishedVersion


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