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dc.creatorMargetić, Aleksandra
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:52:24Z
dc.date.available2019-01-30T17:52:24Z
dc.date.issued2016
dc.identifier.issn0352-5139
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2048
dc.description.abstractCell wall invertase (CWI) from Saccharomyces cerevisiae was immobilized in polyacrylamide hydrogels. The aim of the development of a new biocatalyst was to obtain an improved enzyme for invert sugar production. The monomer concentration and enzyme amount in the immobilizate were optimized, and the obtained biocatalyst had an enzyme activity of 138 +/- 6 IU g(-1). The pH and temperature optima were 4.0 and 70 degrees C, respectively. The stability of immobilized enzyme was determined at several temperatures in the absence of substrate and the half-life obtained at 50 degrees C was 81 days, at 60 degrees C, 128 min and at 70 degrees C, 1.24 min. The biocatalyst was tested at low pH values, 3.0 and 3.5 were tested, and showed great stability. The KM values were 34.1 +/- 1.7 and 126.2 +/- 6.3 mM for free and immobilized CWI, respectively. The activation energies were 37.7 and 23.0 kJ mol(-1) for free and immobilized CWI, respectively. Cell wall invertase immobilized in polyacrylamide hydrogel (CWI-PAA) was tested for the production of high concentrated invert sugar in a batch and a packed bed reactor. After five days of continuous process, the quality and characteristics of the produced invert sugar remained unchanged.en
dc.publisherSerbian Chemical Soc, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectimmobilizationen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectenzymeen
dc.subjectstabilityen
dc.subjectCWI-PAAen
dc.titleImmobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar productionen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractМаргетић, Aлександра; Вујциц, Зоран М.;
dc.citation.volume81
dc.citation.issue12
dc.citation.spage1359
dc.citation.epage1369
dc.citation.other81(12): 1359-1369
dc.citation.rankM23
dc.identifier.doi10.2298/JSC160720094M
dc.identifier.rcubConv_3670
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/8476/2046.pdf
dc.identifier.scopus2-s2.0-85009770680
dc.identifier.wos000392946100003
dc.type.versionpublishedVersion


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