CER - Central Repository
Institute of Chemistry, Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   Central Repository
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
  •   Central Repository
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production

Thumbnail
2016
2046.pdf (340.9Kb)
Authors
Margetić, Aleksandra
Vujčić, Zoran
Article (Published version)
Metadata
Show full item record
Abstract
Cell wall invertase (CWI) from Saccharomyces cerevisiae was immobilized in polyacrylamide hydrogels. The aim of the development of a new biocatalyst was to obtain an improved enzyme for invert sugar production. The monomer concentration and enzyme amount in the immobilizate were optimized, and the obtained biocatalyst had an enzyme activity of 138 +/- 6 IU g(-1). The pH and temperature optima were 4.0 and 70 degrees C, respectively. The stability of immobilized enzyme was determined at several temperatures in the absence of substrate and the half-life obtained at 50 degrees C was 81 days, at 60 degrees C, 128 min and at 70 degrees C, 1.24 min. The biocatalyst was tested at low pH values, 3.0 and 3.5 were tested, and showed great stability. The KM values were 34.1 +/- 1.7 and 126.2 +/- 6.3 mM for free and immobilized CWI, respectively. The activation energies were 37.7 and 23.0 kJ mol(-1) for free and immobilized CWI, respectively. Cell wall invertase immobilized in polyacrylamide hydro...gel (CWI-PAA) was tested for the production of high concentrated invert sugar in a batch and a packed bed reactor. After five days of continuous process, the quality and characteristics of the produced invert sugar remained unchanged.

Keywords:
immobilization / Saccharomyces cerevisiae / enzyme / stability / CWI-PAA
Source:
Journal of the Serbian Chemical Society, 2016, 81, 12, 1359-1369
Publisher:
  • Serbian Chemical Soc, Belgrade
Projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)

DOI: 10.2298/JSC160720094M

ISSN: 0352-5139

WoS: 000392946100003

Scopus: 2-s2.0-85009770680
[ Google Scholar ]
1
2
URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2048
Collections
  • Radovi istraživača / Researchers' publications
Institution
IHTM

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

OpenAIRERCUB