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dc.creatorMarinković, Vesna
dc.creatorRankovic-Janevski, Milica
dc.creatorSpasić, Snežana
dc.creatorNikolić-Kokić, Aleksandra
dc.creatorLugonja, Nikoleta
dc.creatorĐurović, Dijana
dc.creatorMiletić, Srđan
dc.creatorVrvić, Miroslav
dc.creatorSpasojević, Ivan
dc.date.accessioned2019-01-30T17:51:54Z
dc.date.available2019-01-30T17:51:54Z
dc.date.issued2016
dc.identifier.issn0277-2116
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2025
dc.description.abstractObjectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.en
dc.publisherLippincott Williams & Wilkins, Philadelphia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173014/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Pediatric Gastroenterology and Nutrition
dc.subjectascorbateen
dc.subjectglutathioneen
dc.subjecthydroxyl radicalen
dc.subjectsuperoxide dismutaseen
dc.subjecturateen
dc.titleAntioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storageen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЛугоња, Николета; Милетић, Срђан; Врвиц, Мирослав М; Николиц-Кокиц, Aлександра; Дјуровиц, Дијана; Ранковиц-Јаневски, Милица; Маринковиц, Весна; Спасојевиц, Иван; Спасић, Снежана;
dc.citation.volume62
dc.citation.issue6
dc.citation.spage901
dc.citation.epage906
dc.citation.other62(6): 901-906
dc.citation.rankM21
dc.identifier.pmid26794491
dc.identifier.doi10.1097/MPG.0000000000001090
dc.identifier.rcubConv_3547
dc.identifier.scopus2-s2.0-84970015194
dc.identifier.wos000376560600029
dc.type.versionpublishedVersion


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