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Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage

Authorized Users Only
2016
Authors
Marinković, Vesna
Rankovic-Janevski, Milica
Spasić, Snežana
Nikolić-Kokić, Aleksandra
Lugonja, Nikoleta
Đurović, Dijana
Miletić, Srđan
Vrvić, Miroslav
Spasojević, Ivan
Article (Published version)
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Abstract
Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of ...raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.

Keywords:
ascorbate / glutathione / hydroxyl radical / superoxide dismutase / urate
Source:
Journal of Pediatric Gastroenterology and Nutrition, 2016, 62, 6, 901-906
Publisher:
  • Lippincott Williams & Wilkins, Philadelphia
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
  • Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-173014)

DOI: 10.1097/MPG.0000000000001090

ISSN: 0277-2116

PubMed: 26794491

WoS: 000376560600029

Scopus: 2-s2.0-84970015194
[ Google Scholar ]
35
28
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/2025
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Marinković, Vesna
AU  - Rankovic-Janevski, Milica
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Đurović, Dijana
AU  - Miletić, Srđan
AU  - Vrvić, Miroslav
AU  - Spasojević, Ivan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2025
AB  - Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Pediatric Gastroenterology and Nutrition
T1  - Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage
VL  - 62
IS  - 6
SP  - 901
EP  - 906
DO  - 10.1097/MPG.0000000000001090
ER  - 
@article{
author = "Marinković, Vesna and Rankovic-Janevski, Milica and Spasić, Snežana and Nikolić-Kokić, Aleksandra and Lugonja, Nikoleta and Đurović, Dijana and Miletić, Srđan and Vrvić, Miroslav and Spasojević, Ivan",
year = "2016",
abstract = "Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
title = "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage",
volume = "62",
number = "6",
pages = "901-906",
doi = "10.1097/MPG.0000000000001090"
}
Marinković, V., Rankovic-Janevski, M., Spasić, S., Nikolić-Kokić, A., Lugonja, N., Đurović, D., Miletić, S., Vrvić, M.,& Spasojević, I.. (2016). Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition
Lippincott Williams & Wilkins, Philadelphia., 62(6), 901-906.
https://doi.org/10.1097/MPG.0000000000001090
Marinković V, Rankovic-Janevski M, Spasić S, Nikolić-Kokić A, Lugonja N, Đurović D, Miletić S, Vrvić M, Spasojević I. Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition. 2016;62(6):901-906.
doi:10.1097/MPG.0000000000001090 .
Marinković, Vesna, Rankovic-Janevski, Milica, Spasić, Snežana, Nikolić-Kokić, Aleksandra, Lugonja, Nikoleta, Đurović, Dijana, Miletić, Srđan, Vrvić, Miroslav, Spasojević, Ivan, "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage" in Journal of Pediatric Gastroenterology and Nutrition, 62, no. 6 (2016):901-906,
https://doi.org/10.1097/MPG.0000000000001090 . .

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