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dc.creatorIgnjatović, Nenad
dc.creatorPenov-Gasi, Katarina M.
dc.creatorWu, Victoria M.
dc.creatorAjduković, Jovana J.
dc.creatorKojić, Vesna V.
dc.creatorVasiljević-Radović, Dana
dc.creatorKuzmanovic, Maja
dc.creatorUskokovicć, Vuk
dc.creatorUskoković, Dragan P.
dc.date.accessioned2019-01-30T17:51:17Z
dc.date.available2019-01-30T17:51:17Z
dc.date.issued2016
dc.identifier.issn0927-7765
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1996
dc.description.abstractIn an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17 beta-hydroxy-17 alpha-picolyl-androst-5-en-3 beta-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. H-1 NMR and C-13 NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d(50) = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46 +/- 2%), while simultaneously preserving high viability (83 +/- 3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172021/RS//
dc.relationUnited States National Institutes of Health - R00-DE021416
dc.rightsrestrictedAccess
dc.sourceColloids and Surfaces B-Biointerfaces
dc.subjectAndrostaneen
dc.subjectChitosanen
dc.subjectHydroxyapatiteen
dc.subjectLung canceren
dc.subjectNanoparticleen
dc.subjectPLGAen
dc.titleSelective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitoren
dc.typearticle
dc.rights.licenseARR
dcterms.abstractУскоковицћ, Вук; Ускоковић, Драган П.; Игњатовић, Ненад Л.; Пенов-Гаси, Катарина М.; Wу, Вицториа М.; Aјдуковић, Јована Ј.; Којиц, Весна В.; Васиљевић-Радовић, Дана; Кузмановиц, Маја;
dc.citation.volume148
dc.citation.spage629
dc.citation.epage639
dc.citation.other148: 629-639
dc.citation.rankM21
dc.identifier.pmid27694053
dc.identifier.doi10.1016/j.colsurfb.2016.09.041
dc.identifier.rcubConv_3628
dc.identifier.scopus2-s2.0-84989184184
dc.identifier.wos000388248500073
dc.type.versionpublishedVersion


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