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dc.creatorŠokarda Slavić, Marinela
dc.creatorPešić, Milja
dc.creatorVujčić, Zoran
dc.creatorBožić, Nataša
dc.date.accessioned2019-01-30T17:50:40Z
dc.date.available2019-01-30T17:50:40Z
dc.date.issued2016
dc.identifier.issn0175-7598
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1965
dc.description.abstractalpha-Amylase from Bacillus licheniformis ATCC 9945a (BliAmy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of BliAmy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), BliAmy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. BliAmy presents interesting performances on highly concentrated solid starch and could be of value for starch-consuming industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationInternational Centre for Genetic Engineering and Biotechnology (ICGEB) [CRP/YUG11-02]
dc.rightsrestrictedAccess
dc.sourceApplied Microbiology and Biotechnology
dc.subjectBacillus sp.en
dc.subjectAmylaseen
dc.subjectFed-batchen
dc.subjectPurificationen
dc.subjectStatistical experimental designen
dc.subjectRaw starch degradationen
dc.titleOvercoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylaseen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујциц, Зоран; Шокарда Славић, Маринела; Божић, Наташа; Песиц, Миља;
dc.citation.volume100
dc.citation.issue6
dc.citation.spage2709
dc.citation.epage2719
dc.citation.other100(6): 2709-2719
dc.citation.rankM21
dc.identifier.doi10.1007/s00253-015-7101-4
dc.identifier.rcubConv_3500
dc.identifier.scopus2-s2.0-84959107103
dc.identifier.wos000371244300018
dc.type.versionpublishedVersion


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