Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation
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2016
Authors
Vajdle, OlgaGuzsvány, Valéria

Škorić, Dušan
Anojčić, Jasmina

Jovanov, Pavle
Avramov Ivić, Milka

Csanadi, Janos
Kónya, Zoltán

Petrović, Slobodan D.
Bobrowski, Andrzej
Article (Published version)

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Erythromycin, a macrolide antibiotic, has similar antimicrobial spectrum to penicillin and it is widely used, especially in the treatment of patients who are allergic to penicillin. In this work, the application of a renewable silver-amalgam film electrode (Hg(Ag)FE) for the characterization and determination of erythromycin ethylsuccinate (EES), a widely used esterified form of this antibiotic, by means of cyclic voltammetry (CV) and square wave voltammetry (SWV) is presented. In the aqueous Britton-Robinson buffer (pH 5.0-9.0) that served as the supporting electrolyte, one reduction peak of EES was observed in the investigated potential range between -0.75 V and -1.80 V vs SCE, with peak potential maxima ranging from -1.59 V to -1.70 V, which strongly depended on the applied pH, as did the peak shape. For the analytical purposes the pH of 7.0 was selected, since in this electrolyte the EES peak was well-shaped and separated from the background current of the supporting electrolyte in... both cases; in the direct cathodic SWV and in the case of square wave adsorptive stripping voltammetry (SW-AdSV). It was established, by the E-p-pH correlation, that protons strongly influenced the electrochemical reduction of EES. The CVs recorded between 0.025-0.50 V s (1) at pH 7.0 confirmed that the electrode reaction is adsorption-controlled. Based on the series of H-1 NMR measurements it is proved that the tertiary amino group of EES is mainly in its protonated form at pH 7.0 which may lead, at appropriate accumulation potential and time, to the favored adsorption of the target ionic form of the analyte improving on such way the sensitivity of the SW-AdSV method. The optimized procedures resulted in stable SWV responses with good linear correlation in the EES concentration range from 4.53 to 29.8 mu g mL (1) (LOD = 1.36 mu g mL (1)), and from 0.69 mu g mL (1) to 2.44 mu g mL (1) (LOD 0.21 mu g mL (1)) in the case of optimized SW-AdSV. The relative standard deviation is below 1.5%. The reliability of the elaborated procedures and thus the accuracy of the obtained results were validated by comparing them with those obtained by means of HPLC-DAD measurements. The direct cathodic SWV method was successfully applied for the determination of EES in the pharmaceutical preparation Eritromicin (R), while SW-AdSV was applied in the case of the spiked urine sample. In both cases, the standard addition method was used.
Keywords:
erythromycin ethylsuccinate / square wave adsorptive stripping / voltammetry renewable silver amalgam-film electrode / urine sample / pharmaceutical preparationSource:
Electrochimica Acta, 2016, 191, 44-54Publisher:
- Oxford : Pergamon-Elsevier Science Ltd
Funding / projects:
- Sustainable and green chemistry approach for environmental friendly analytical methods and energy storage (RS-172012)
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- CEEPUSIII - CZ-0212-09-1516
Note:
- The peer-reviewed version: https://cer.ihtm.bg.ac.rs/handle/123456789/4319
Related info:
DOI: 10.1016/j.electacta.2015.12.207
ISSN: 0013-4686
WoS: 000371143200006
Scopus: 2-s2.0-84955469852
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IHTMTY - JOUR AU - Vajdle, Olga AU - Guzsvány, Valéria AU - Škorić, Dušan AU - Anojčić, Jasmina AU - Jovanov, Pavle AU - Avramov Ivić, Milka AU - Csanadi, Janos AU - Kónya, Zoltán AU - Petrović, Slobodan D. AU - Bobrowski, Andrzej PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1964 AB - Erythromycin, a macrolide antibiotic, has similar antimicrobial spectrum to penicillin and it is widely used, especially in the treatment of patients who are allergic to penicillin. In this work, the application of a renewable silver-amalgam film electrode (Hg(Ag)FE) for the characterization and determination of erythromycin ethylsuccinate (EES), a widely used esterified form of this antibiotic, by means of cyclic voltammetry (CV) and square wave voltammetry (SWV) is presented. In the aqueous Britton-Robinson buffer (pH 5.0-9.0) that served as the supporting electrolyte, one reduction peak of EES was observed in the investigated potential range between -0.75 V and -1.80 V vs SCE, with peak potential maxima ranging from -1.59 V to -1.70 V, which strongly depended on the applied pH, as did the peak shape. For the analytical purposes the pH of 7.0 was selected, since in this electrolyte the EES peak was well-shaped and separated from the background current of the supporting electrolyte in both cases; in the direct cathodic SWV and in the case of square wave adsorptive stripping voltammetry (SW-AdSV). It was established, by the E-p-pH correlation, that protons strongly influenced the electrochemical reduction of EES. The CVs recorded between 0.025-0.50 V s (1) at pH 7.0 confirmed that the electrode reaction is adsorption-controlled. Based on the series of H-1 NMR measurements it is proved that the tertiary amino group of EES is mainly in its protonated form at pH 7.0 which may lead, at appropriate accumulation potential and time, to the favored adsorption of the target ionic form of the analyte improving on such way the sensitivity of the SW-AdSV method. The optimized procedures resulted in stable SWV responses with good linear correlation in the EES concentration range from 4.53 to 29.8 mu g mL (1) (LOD = 1.36 mu g mL (1)), and from 0.69 mu g mL (1) to 2.44 mu g mL (1) (LOD 0.21 mu g mL (1)) in the case of optimized SW-AdSV. The relative standard deviation is below 1.5%. The reliability of the elaborated procedures and thus the accuracy of the obtained results were validated by comparing them with those obtained by means of HPLC-DAD measurements. The direct cathodic SWV method was successfully applied for the determination of EES in the pharmaceutical preparation Eritromicin (R), while SW-AdSV was applied in the case of the spiked urine sample. In both cases, the standard addition method was used. PB - Oxford : Pergamon-Elsevier Science Ltd T2 - Electrochimica Acta T1 - Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation VL - 191 SP - 44 EP - 54 DO - 10.1016/j.electacta.2015.12.207 ER -
@article{ author = "Vajdle, Olga and Guzsvány, Valéria and Škorić, Dušan and Anojčić, Jasmina and Jovanov, Pavle and Avramov Ivić, Milka and Csanadi, Janos and Kónya, Zoltán and Petrović, Slobodan D. and Bobrowski, Andrzej", year = "2016", abstract = "Erythromycin, a macrolide antibiotic, has similar antimicrobial spectrum to penicillin and it is widely used, especially in the treatment of patients who are allergic to penicillin. In this work, the application of a renewable silver-amalgam film electrode (Hg(Ag)FE) for the characterization and determination of erythromycin ethylsuccinate (EES), a widely used esterified form of this antibiotic, by means of cyclic voltammetry (CV) and square wave voltammetry (SWV) is presented. In the aqueous Britton-Robinson buffer (pH 5.0-9.0) that served as the supporting electrolyte, one reduction peak of EES was observed in the investigated potential range between -0.75 V and -1.80 V vs SCE, with peak potential maxima ranging from -1.59 V to -1.70 V, which strongly depended on the applied pH, as did the peak shape. For the analytical purposes the pH of 7.0 was selected, since in this electrolyte the EES peak was well-shaped and separated from the background current of the supporting electrolyte in both cases; in the direct cathodic SWV and in the case of square wave adsorptive stripping voltammetry (SW-AdSV). It was established, by the E-p-pH correlation, that protons strongly influenced the electrochemical reduction of EES. The CVs recorded between 0.025-0.50 V s (1) at pH 7.0 confirmed that the electrode reaction is adsorption-controlled. Based on the series of H-1 NMR measurements it is proved that the tertiary amino group of EES is mainly in its protonated form at pH 7.0 which may lead, at appropriate accumulation potential and time, to the favored adsorption of the target ionic form of the analyte improving on such way the sensitivity of the SW-AdSV method. The optimized procedures resulted in stable SWV responses with good linear correlation in the EES concentration range from 4.53 to 29.8 mu g mL (1) (LOD = 1.36 mu g mL (1)), and from 0.69 mu g mL (1) to 2.44 mu g mL (1) (LOD 0.21 mu g mL (1)) in the case of optimized SW-AdSV. The relative standard deviation is below 1.5%. The reliability of the elaborated procedures and thus the accuracy of the obtained results were validated by comparing them with those obtained by means of HPLC-DAD measurements. The direct cathodic SWV method was successfully applied for the determination of EES in the pharmaceutical preparation Eritromicin (R), while SW-AdSV was applied in the case of the spiked urine sample. In both cases, the standard addition method was used.", publisher = "Oxford : Pergamon-Elsevier Science Ltd", journal = "Electrochimica Acta", title = "Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation", volume = "191", pages = "44-54", doi = "10.1016/j.electacta.2015.12.207" }
Vajdle, O., Guzsvány, V., Škorić, D., Anojčić, J., Jovanov, P., Avramov Ivić, M., Csanadi, J., Kónya, Z., Petrović, S. D.,& Bobrowski, A.. (2016). Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation. in Electrochimica Acta Oxford : Pergamon-Elsevier Science Ltd., 191, 44-54. https://doi.org/10.1016/j.electacta.2015.12.207
Vajdle O, Guzsvány V, Škorić D, Anojčić J, Jovanov P, Avramov Ivić M, Csanadi J, Kónya Z, Petrović SD, Bobrowski A. Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation. in Electrochimica Acta. 2016;191:44-54. doi:10.1016/j.electacta.2015.12.207 .
Vajdle, Olga, Guzsvány, Valéria, Škorić, Dušan, Anojčić, Jasmina, Jovanov, Pavle, Avramov Ivić, Milka, Csanadi, Janos, Kónya, Zoltán, Petrović, Slobodan D., Bobrowski, Andrzej, "Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation" in Electrochimica Acta, 191 (2016):44-54, https://doi.org/10.1016/j.electacta.2015.12.207 . .