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Management of Diabetic Bacterial Foot Infections with Organic Extracts of Liverwort Marchantia debilis from Cameroon

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2016
1934578x1601100938.pdf (341.3Kb)
Authors
Yongabi, Kenneth Anchang
Novaković, Miroslav
Bukvicki, Danka
Reeb, Catherine
Asakawa, Yoshinori
Article (Published version)
Metadata
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Abstract
Diabetic bacterial foot infections (DBFIs) are limb-threatening complications in patients with diabetes mellitus, accounting for 50% of diabetes related lower limb amputations in developing countries, representing approximately 20 percent of all diabetes-related hospital admissions with significant healthcare-related costs involved. The widespread problem of bacterial resistance to most commonly used antibiotics places a huge economic burden on the healthcare system, with both increased morbidity and mortality among diabetic patients with foot infections. In this study, the antibacterial activity of organic extracts of the fresh liverwort Marchantia debilis from the North West Region of Cameroon is reported. An exit pool system, where patients presenting with DBFIs consented to be involved in the use of phytomedicines, after long term treatment of ulcers with antibiotics and not yielding significant long term benefit, presented themselves at the Phytobiotechnology Research clinic (PRF).... Continuous culture of swabs from foot and toe wounds from 30 infected patients on nutrient agar and MacConkey agars in triplicate as well as Gram stain microscopy, revealed the presence of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and Bacillus species. Light petroleum and methanol extracts of the whole bryophyte plants at 100% concentration were tested. In vitro inhibition of the tested bacterial isolates from the diabetic foot ulcers by M debilis was observed only with the light petroleum extract. No inhibition by the extracts was observed for the Pseudomonas aeruginosa isolate. The light petroleum extract of M debilis was formulated into a petroleum oil based cream named BryoCream (TM). This was administered to 20 of the patients with 90% cure rate in a three week time period. The main nonpolar components were determined by GCMS as lepidozene and beta-barbatene, and by NMR. as stigmasterol and beta-sitosterol. In conclusion, nonpolar extracts from bryophytes from Cameroon could, potentially, be used to treat diabetic bacterial foot infections.

Keywords:
Marchantia debilis / Liverwort / Diabetic / Foot / Bacteria / Infection
Source:
Natural Product Communications, 2016, 11, 9, 1333-1336
Publisher:
  • Natural Products Inc, Westerville
Funding / projects:
  • Phytobiotechnology Research Foundation of Cameroon
  • Matsumae International Foundation

ISSN: 1934-578X

WoS: 000384513600038

[ Google Scholar ]
2
Handle
https://hdl.handle.net/21.15107/rcub_cer_1926
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/1926
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Yongabi, Kenneth Anchang
AU  - Novaković, Miroslav
AU  - Bukvicki, Danka
AU  - Reeb, Catherine
AU  - Asakawa, Yoshinori
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1926
AB  - Diabetic bacterial foot infections (DBFIs) are limb-threatening complications in patients with diabetes mellitus, accounting for 50% of diabetes related lower limb amputations in developing countries, representing approximately 20 percent of all diabetes-related hospital admissions with significant healthcare-related costs involved. The widespread problem of bacterial resistance to most commonly used antibiotics places a huge economic burden on the healthcare system, with both increased morbidity and mortality among diabetic patients with foot infections. In this study, the antibacterial activity of organic extracts of the fresh liverwort Marchantia debilis from the North West Region of Cameroon is reported. An exit pool system, where patients presenting with DBFIs consented to be involved in the use of phytomedicines, after long term treatment of ulcers with antibiotics and not yielding significant long term benefit, presented themselves at the Phytobiotechnology Research clinic (PRF). Continuous culture of swabs from foot and toe wounds from 30 infected patients on nutrient agar and MacConkey agars in triplicate as well as Gram stain microscopy, revealed the presence of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and Bacillus species. Light petroleum and methanol extracts of the whole bryophyte plants at 100% concentration were tested. In vitro inhibition of the tested bacterial isolates from the diabetic foot ulcers by M debilis was observed only with the light petroleum extract. No inhibition by the extracts was observed for the Pseudomonas aeruginosa isolate. The light petroleum extract of M debilis was formulated into a petroleum oil based cream named BryoCream (TM). This was administered to 20 of the patients with 90% cure rate in a three week time period. The main nonpolar components were determined by GCMS as lepidozene and beta-barbatene, and by NMR. as stigmasterol and beta-sitosterol. In conclusion, nonpolar extracts from bryophytes from Cameroon could, potentially, be used to treat diabetic bacterial foot infections.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Management of Diabetic Bacterial Foot Infections with Organic Extracts of Liverwort Marchantia debilis from Cameroon
VL  - 11
IS  - 9
SP  - 1333
EP  - 1336
UR  - https://hdl.handle.net/21.15107/rcub_cer_1926
ER  - 
@article{
author = "Yongabi, Kenneth Anchang and Novaković, Miroslav and Bukvicki, Danka and Reeb, Catherine and Asakawa, Yoshinori",
year = "2016",
abstract = "Diabetic bacterial foot infections (DBFIs) are limb-threatening complications in patients with diabetes mellitus, accounting for 50% of diabetes related lower limb amputations in developing countries, representing approximately 20 percent of all diabetes-related hospital admissions with significant healthcare-related costs involved. The widespread problem of bacterial resistance to most commonly used antibiotics places a huge economic burden on the healthcare system, with both increased morbidity and mortality among diabetic patients with foot infections. In this study, the antibacterial activity of organic extracts of the fresh liverwort Marchantia debilis from the North West Region of Cameroon is reported. An exit pool system, where patients presenting with DBFIs consented to be involved in the use of phytomedicines, after long term treatment of ulcers with antibiotics and not yielding significant long term benefit, presented themselves at the Phytobiotechnology Research clinic (PRF). Continuous culture of swabs from foot and toe wounds from 30 infected patients on nutrient agar and MacConkey agars in triplicate as well as Gram stain microscopy, revealed the presence of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and Bacillus species. Light petroleum and methanol extracts of the whole bryophyte plants at 100% concentration were tested. In vitro inhibition of the tested bacterial isolates from the diabetic foot ulcers by M debilis was observed only with the light petroleum extract. No inhibition by the extracts was observed for the Pseudomonas aeruginosa isolate. The light petroleum extract of M debilis was formulated into a petroleum oil based cream named BryoCream (TM). This was administered to 20 of the patients with 90% cure rate in a three week time period. The main nonpolar components were determined by GCMS as lepidozene and beta-barbatene, and by NMR. as stigmasterol and beta-sitosterol. In conclusion, nonpolar extracts from bryophytes from Cameroon could, potentially, be used to treat diabetic bacterial foot infections.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Management of Diabetic Bacterial Foot Infections with Organic Extracts of Liverwort Marchantia debilis from Cameroon",
volume = "11",
number = "9",
pages = "1333-1336",
url = "https://hdl.handle.net/21.15107/rcub_cer_1926"
}
Yongabi, K. A., Novaković, M., Bukvicki, D., Reeb, C.,& Asakawa, Y.. (2016). Management of Diabetic Bacterial Foot Infections with Organic Extracts of Liverwort Marchantia debilis from Cameroon. in Natural Product Communications
Natural Products Inc, Westerville., 11(9), 1333-1336.
https://hdl.handle.net/21.15107/rcub_cer_1926
Yongabi KA, Novaković M, Bukvicki D, Reeb C, Asakawa Y. Management of Diabetic Bacterial Foot Infections with Organic Extracts of Liverwort Marchantia debilis from Cameroon. in Natural Product Communications. 2016;11(9):1333-1336.
https://hdl.handle.net/21.15107/rcub_cer_1926 .
Yongabi, Kenneth Anchang, Novaković, Miroslav, Bukvicki, Danka, Reeb, Catherine, Asakawa, Yoshinori, "Management of Diabetic Bacterial Foot Infections with Organic Extracts of Liverwort Marchantia debilis from Cameroon" in Natural Product Communications, 11, no. 9 (2016):1333-1336,
https://hdl.handle.net/21.15107/rcub_cer_1926 .

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