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Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1
GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin, showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this study showed the considerable biological potential of H. orphanidis considering free radica...ls and various pathogenic strains, including the wild type of P. aeruginosa.
TY - JOUR
AU - Mileski, Ksenija S
AU - Ciric, Ana D
AU - Trifunović, Snežana
AU - Ristic, Mihailo S
AU - Soković, Marina
AU - Matevski, Vlado S
AU - Tešević, Vele
AU - Jadranin, Milka
AU - Marin, Petar D.
AU - Dzamic, Ana M
PY - 2016
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1923
AB - GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin, showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this study showed the considerable biological potential of H. orphanidis considering free radicals and various pathogenic strains, including the wild type of P. aeruginosa.
PB - Royal Soc Chemistry, Cambridge
T2 - Food & Function
T1 - Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1
VL - 7
IS - 9
SP - 4061
EP - 4074
DO - 10.1039/c6fo01018k
ER -
@article{
author = "Mileski, Ksenija S and Ciric, Ana D and Trifunović, Snežana and Ristic, Mihailo S and Soković, Marina and Matevski, Vlado S and Tešević, Vele and Jadranin, Milka and Marin, Petar D. and Dzamic, Ana M",
year = "2016",
abstract = "GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin, showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this study showed the considerable biological potential of H. orphanidis considering free radicals and various pathogenic strains, including the wild type of P. aeruginosa.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1",
volume = "7",
number = "9",
pages = "4061-4074",
doi = "10.1039/c6fo01018k"
}
Mileski, K. S., Ciric, A. D., Trifunović, S., Ristic, M. S., Soković, M., Matevski, V. S., Tešević, V., Jadranin, M., Marin, P. D.,& Dzamic, A. M.. (2016). Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1. in Food & Function
Royal Soc Chemistry, Cambridge., 7(9), 4061-4074.
https://doi.org/10.1039/c6fo01018k
Mileski KS, Ciric AD, Trifunović S, Ristic MS, Soković M, Matevski VS, Tešević V, Jadranin M, Marin PD, Dzamic AM. Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1. in Food & Function. 2016;7(9):4061-4074.
doi:10.1039/c6fo01018k .
Mileski, Ksenija S, Ciric, Ana D, Trifunović, Snežana, Ristic, Mihailo S, Soković, Marina, Matevski, Vlado S, Tešević, Vele, Jadranin, Milka, Marin, Petar D., Dzamic, Ana M, "Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1" in Food & Function, 7, no. 9 (2016):4061-4074,
https://doi.org/10.1039/c6fo01018k . .
