Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1
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2016
Authors
Mileski, Ksenija SCiric, Ana D
Trifunović, Snežana

Ristic, Mihailo S
Soković, Marina

Matevski, Vlado S
Tešević, Vele

Jadranin, Milka

Marin, Petar D.

Dzamic, Ana M
Article (Published version)

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Show full item recordAbstract
GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin, showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this study showed the considerable biological potential of H. orphanidis considering free radica...ls and various pathogenic strains, including the wild type of P. aeruginosa.
Source:
Food & Function, 2016, 7, 9, 4061-4074Publisher:
- Royal Soc Chemistry, Cambridge
Projects:
- Micromorphological, phytochemical and molecular investigations of plants - systematic, ecological and applicative aspects (RS-173029)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-173032)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
Note:
- The peer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/3130
DOI: 10.1039/c6fo01018k
ISSN: 2042-6496
PubMed: 27713984