Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode
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2016
Authors
Lović, Jelena
Trišović, Nemanja

Antanasijevic, Jelena
Nikolić, Nebojša D.

Stevanović, Sanja

Mijin, Dušan

Vukovic, Dragan

Mladenović, Aleksandar R.

Petrović, Slobodan D.
Avramov Ivić, Milka

Article (Published version)

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Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC...-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.
Keywords:
Sildenafil citrate / Au/Cys electrode / Human serum analysis / Surface microscopic analysisSource:
Journal of Electroanalytical Chemistry, 2016, 782, 103-107Publisher:
- Elsevier
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- New approach in designing materials for energy conversion and energy storage systems (RS-172060)
Note:
- The peer-reviewed version: https://cer.ihtm.bg.ac.rs/handle/123456789/4310
Related info:
DOI: 10.1016/j.jelechem.2016.10.022
ISSN: 1572-6657
WoS: 000390510000015
Scopus: 2-s2.0-84992195164
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IHTMTY - JOUR AU - Lović, Jelena AU - Trišović, Nemanja AU - Antanasijevic, Jelena AU - Nikolić, Nebojša D. AU - Stevanović, Sanja AU - Mijin, Dušan AU - Vukovic, Dragan AU - Mladenović, Aleksandar R. AU - Petrović, Slobodan D. AU - Avramov Ivić, Milka PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1902 AB - Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte. PB - Elsevier T2 - Journal of Electroanalytical Chemistry T1 - Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode VL - 782 SP - 103 EP - 107 DO - 10.1016/j.jelechem.2016.10.022 ER -
@article{ author = "Lović, Jelena and Trišović, Nemanja and Antanasijevic, Jelena and Nikolić, Nebojša D. and Stevanović, Sanja and Mijin, Dušan and Vukovic, Dragan and Mladenović, Aleksandar R. and Petrović, Slobodan D. and Avramov Ivić, Milka", year = "2016", abstract = "Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.", publisher = "Elsevier", journal = "Journal of Electroanalytical Chemistry", title = "Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode", volume = "782", pages = "103-107", doi = "10.1016/j.jelechem.2016.10.022" }
Lović, J., Trišović, N., Antanasijevic, J., Nikolić, N. D., Stevanović, S., Mijin, D., Vukovic, D., Mladenović, A. R., Petrović, S. D.,& Avramov Ivić, M.. (2016). Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode. in Journal of Electroanalytical Chemistry Elsevier., 782, 103-107. https://doi.org/10.1016/j.jelechem.2016.10.022
Lović J, Trišović N, Antanasijevic J, Nikolić ND, Stevanović S, Mijin D, Vukovic D, Mladenović AR, Petrović SD, Avramov Ivić M. Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode. in Journal of Electroanalytical Chemistry. 2016;782:103-107. doi:10.1016/j.jelechem.2016.10.022 .
Lović, Jelena, Trišović, Nemanja, Antanasijevic, Jelena, Nikolić, Nebojša D., Stevanović, Sanja, Mijin, Dušan, Vukovic, Dragan, Mladenović, Aleksandar R., Petrović, Slobodan D., Avramov Ivić, Milka, "Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode" in Journal of Electroanalytical Chemistry, 782 (2016):103-107, https://doi.org/10.1016/j.jelechem.2016.10.022 . .