Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC...-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.
TY - JOUR
AU - Lović, Jelena
AU - Trišović, Nemanja
AU - Antanasijevic, Jelena
AU - Nikolić, Nebojša D.
AU - Stevanović, Sanja
AU - Mijin, Dušan
AU - Vukovic, Dragan
AU - Mladenović, Aleksandar R.
AU - Petrović, Slobodan D.
AU - Avramov Ivić, Milka
PY - 2016
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1902
AB - Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.
PB - Elsevier
T2 - Journal of Electroanalytical Chemistry
T1 - Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode
VL - 782
SP - 103
EP - 107
DO - 10.1016/j.jelechem.2016.10.022
ER -
@article{
author = "Lović, Jelena and Trišović, Nemanja and Antanasijevic, Jelena and Nikolić, Nebojša D. and Stevanović, Sanja and Mijin, Dušan and Vukovic, Dragan and Mladenović, Aleksandar R. and Petrović, Slobodan D. and Avramov Ivić, Milka",
year = "2016",
abstract = "Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena (R) tablets is determined by square wave voltammetry (SWV) in 0.1 M H2SO4 (harmful for human serum) at gold electrode in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.5,1) mu M and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (1 x 10(-3),1 x 10(-2), 0.05, 0.1) mu M. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena (R) tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO3 (suitable for human liquids) in a range: (1 x 10(-3),1 x 10(-2), 0.1, 0.2, 03) mu M. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.",
publisher = "Elsevier",
journal = "Journal of Electroanalytical Chemistry",
title = "Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode",
volume = "782",
pages = "103-107",
doi = "10.1016/j.jelechem.2016.10.022"
}
Lović, J., Trišović, N., Antanasijevic, J., Nikolić, N. D., Stevanović, S., Mijin, D., Vukovic, D., Mladenović, A. R., Petrović, S. D.,& Avramov Ivić, M.. (2016). Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode. in Journal of Electroanalytical Chemistry
Elsevier., 782, 103-107.
https://doi.org/10.1016/j.jelechem.2016.10.022
Lović J, Trišović N, Antanasijevic J, Nikolić ND, Stevanović S, Mijin D, Vukovic D, Mladenović AR, Petrović SD, Avramov Ivić M. Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode. in Journal of Electroanalytical Chemistry. 2016;782:103-107.
doi:10.1016/j.jelechem.2016.10.022 .
Lović, Jelena, Trišović, Nemanja, Antanasijevic, Jelena, Nikolić, Nebojša D., Stevanović, Sanja, Mijin, Dušan, Vukovic, Dragan, Mladenović, Aleksandar R., Petrović, Slobodan D., Avramov Ivić, Milka, "Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode" in Journal of Electroanalytical Chemistry, 782 (2016):103-107,
https://doi.org/10.1016/j.jelechem.2016.10.022 . .
