The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA. hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA. hy926 cells and initial steps of angiogenesis. In addition, etha...nol extract exerted significant anti-angiogenic effect.
Source:
Bangladesh Journal of Pharmacology, 2016, 11, 3, 741-749
TY - JOUR
AU - Damjanovic, Ana
AU - Zdunić, Gordana
AU - Savikin, Katarina
AU - Mandić, Boris
AU - Jadranin, Milka
AU - Matić, Ivana Z.
AU - Stanojković, Tatjana
PY - 2016
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1901
AB - The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA. hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA. hy926 cells and initial steps of angiogenesis. In addition, ethanol extract exerted significant anti-angiogenic effect.
PB - Bangladesh Pharmacological Soc, Shahbah
T2 - Bangladesh Journal of Pharmacology
T1 - Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis
VL - 11
IS - 3
SP - 741
EP - 749
DO - 10.3329/bjp.v11i3.27103
ER -
@article{
author = "Damjanovic, Ana and Zdunić, Gordana and Savikin, Katarina and Mandić, Boris and Jadranin, Milka and Matić, Ivana Z. and Stanojković, Tatjana",
year = "2016",
abstract = "The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA. hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA. hy926 cells and initial steps of angiogenesis. In addition, ethanol extract exerted significant anti-angiogenic effect.",
publisher = "Bangladesh Pharmacological Soc, Shahbah",
journal = "Bangladesh Journal of Pharmacology",
title = "Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis",
volume = "11",
number = "3",
pages = "741-749",
doi = "10.3329/bjp.v11i3.27103"
}
Damjanovic, A., Zdunić, G., Savikin, K., Mandić, B., Jadranin, M., Matić, I. Z.,& Stanojković, T.. (2016). Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis. in Bangladesh Journal of Pharmacology
Bangladesh Pharmacological Soc, Shahbah., 11(3), 741-749.
https://doi.org/10.3329/bjp.v11i3.27103
Damjanovic A, Zdunić G, Savikin K, Mandić B, Jadranin M, Matić IZ, Stanojković T. Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis. in Bangladesh Journal of Pharmacology. 2016;11(3):741-749.
doi:10.3329/bjp.v11i3.27103 .
Damjanovic, Ana, Zdunić, Gordana, Savikin, Katarina, Mandić, Boris, Jadranin, Milka, Matić, Ivana Z., Stanojković, Tatjana, "Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis" in Bangladesh Journal of Pharmacology, 11, no. 3 (2016):741-749,
https://doi.org/10.3329/bjp.v11i3.27103 . .
