Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis

2016
Authors
Damjanovic, AnaZdunić, Gordana

Savikin, Katarina
Mandić, Boris

Jadranin, Milka

Matić, Ivana Z.

Stanojković, Tatjana

Article (Published version)
Metadata
Show full item recordAbstract
The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA. hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA. hy926 cells and initial steps of angiogenesis. In addition, etha...nol extract exerted significant anti-angiogenic effect.
Source:
Bangladesh Journal of Pharmacology, 2016, 11, 3, 741-749Publisher:
- Bangladesh Pharmacological Soc, Shahbah
Funding / projects:
DOI: 10.3329/bjp.v11i3.27103
ISSN: 1991-007X
WoS: 000390318200027
Scopus: 2-s2.0-84989813691
Collections
Institution/Community
IHTMTY - JOUR AU - Damjanovic, Ana AU - Zdunić, Gordana AU - Savikin, Katarina AU - Mandić, Boris AU - Jadranin, Milka AU - Matić, Ivana Z. AU - Stanojković, Tatjana PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1901 AB - The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA. hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA. hy926 cells and initial steps of angiogenesis. In addition, ethanol extract exerted significant anti-angiogenic effect. PB - Bangladesh Pharmacological Soc, Shahbah T2 - Bangladesh Journal of Pharmacology T1 - Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis VL - 11 IS - 3 SP - 741 EP - 749 DO - 10.3329/bjp.v11i3.27103 ER -
@article{ author = "Damjanovic, Ana and Zdunić, Gordana and Savikin, Katarina and Mandić, Boris and Jadranin, Milka and Matić, Ivana Z. and Stanojković, Tatjana", year = "2016", abstract = "The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA. hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA. hy926 cells and initial steps of angiogenesis. In addition, ethanol extract exerted significant anti-angiogenic effect.", publisher = "Bangladesh Pharmacological Soc, Shahbah", journal = "Bangladesh Journal of Pharmacology", title = "Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis", volume = "11", number = "3", pages = "741-749", doi = "10.3329/bjp.v11i3.27103" }
Damjanovic, A., Zdunić, G., Savikin, K., Mandić, B., Jadranin, M., Matić, I. Z.,& Stanojković, T.. (2016). Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis. in Bangladesh Journal of Pharmacology Bangladesh Pharmacological Soc, Shahbah., 11(3), 741-749. https://doi.org/10.3329/bjp.v11i3.27103
Damjanovic A, Zdunić G, Savikin K, Mandić B, Jadranin M, Matić IZ, Stanojković T. Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis. in Bangladesh Journal of Pharmacology. 2016;11(3):741-749. doi:10.3329/bjp.v11i3.27103 .
Damjanovic, Ana, Zdunić, Gordana, Savikin, Katarina, Mandić, Boris, Jadranin, Milka, Matić, Ivana Z., Stanojković, Tatjana, "Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis" in Bangladesh Journal of Pharmacology, 11, no. 3 (2016):741-749, https://doi.org/10.3329/bjp.v11i3.27103 . .