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dc.creatorApostolovic, Danijela
dc.creatorStanić-Vučinić, Dragana
dc.creatorde, Jongh Harmen H J
dc.creatorde, Jong Govardus A H
dc.creatorMihailović, Jelena
dc.creatorRadosavljević, Jelena
dc.creatorRadibratović, Milica
dc.creatorNordlee, Julie A
dc.creatorBaumert, Joseph L
dc.creatorMilčić, Miloš
dc.creatorTaylor, Steve L
dc.creatorClua, Nuria Garrido
dc.creatorĆirković Veličković, Tanja
dc.creatorKoppelman, Stef J
dc.date.accessioned2019-01-30T17:48:23Z
dc.date.available2019-01-30T17:48:23Z
dc.date.issued2016
dc.identifier.issn2045-2322
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1853
dc.description.abstractConglutins represent the major peanut allergens and are renowned for their resistance to gastrointestinal digestion. Our aim was to characterize the digestion-resistant peptides (DRPs) of conglutins by biochemical and biophysical methods followed by a molecular dynamics simulation in order to better understand the molecular basis of food protein allergenicity. We have mapped proteolysis sites at the N- and C-termini and at a limited internal segment, while other potential proteolysis sites remained unaffected. Molecular dynamics simulation showed that proteolysis only occurred in the vibrant regions of the proteins. DRPs appeared to be conformationally stable as intact conglutins. Also, the overall secondary structure and IgE-binding potency of DRPs was comparable to that of intact conglutins. The stability of conglutins toward gastro-intestinal digestion, combined with the conformational stability of the resulting DRPs provide conditions for optimal exposure to the intestinal immune system, providing an explanation for the extraordinary allergenicity of peanut conglutins.en
dc.publisherNature Publishing Group, London
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171017/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/256716/EU//
dc.rightsopenAccess
dc.sourceScientific Reports
dc.titleConformational stability of digestion-resistant peptides of peanut conglutins reveals the molecular basis of their allergenicityen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractКоппелман, Стеф Ј; де, Јонгх Хармен Х Ј; де, Јонг Говардус A Х; Нордлее, Јулие A; Баумерт, Јосепх Л; Милциц, Милос; Таyлор, Стеве Л; Радибратовић, Милица; Радосављевиц, Јелена; Станиц-Вуциниц, Драгана; Цлуа, Нуриа Гарридо; Aпостоловиц, Данијела; Цирковиц-Велицковиц, Тања; Михаиловиц, Јелена;
dc.citation.volume6
dc.citation.other6:
dc.citation.rankaM21
dc.identifier.pmid27377129
dc.identifier.doi10.1038/srep29249
dc.identifier.rcubConv_3562
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/8290/1851.pdf
dc.identifier.scopus2-s2.0-84977267167
dc.identifier.wos000379265400001
dc.type.versionpublishedVersion


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