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Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases

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2015
1813.pdf (514.8Kb)
Аутори
Lončar, Nikola
Šokarda Slavić, Marinela
Vujčić, Zoran
Božić, Nataša
Чланак у часопису (Објављена верзија)
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Апстракт
Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.
Извор:
Scientific Reports, 2015, 5
Издавач:
  • Nature Publishing Group, London
Пројекти:
  • Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-172048)
  • ICGEB research project [CRP/YUG11-02]

DOI: 10.1038/srep15772

ISSN: 2045-2322

PubMed: 26492875

WoS: 000363430700001

Scopus: 2-s2.0-84945243760
[ Google Scholar ]
2
1
URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1815
Колекције
  • Radovi istraživača / Researchers' publications
Институција
IHTM
TY  - JOUR
AU  - Lončar, Nikola
AU  - Šokarda Slavić, Marinela
AU  - Vujčić, Zoran
AU  - Božić, Nataša
PY  - 2015
UR  - http://cer.ihtm.bg.ac.rs/handle/123456789/1815
AB  - Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases
VL  - 5
DO  - 10.1038/srep15772
ER  - 
@article{
author = "Lončar, Nikola and Šokarda Slavić, Marinela and Vujčić, Zoran and Božić, Nataša",
year = "2015",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/1815",
abstract = "Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases",
volume = "5",
doi = "10.1038/srep15772"
}
Lončar N, Šokarda Slavić M, Vujčić Z, Božić N. Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases. Scientific Reports. 2015;5
Lončar, N., Šokarda Slavić, M., Vujčić, Z.,& Božić, N. (2015). Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases.
Scientific ReportsNature Publishing Group, London., 5.
https://doi.org/10.1038/srep15772
Lončar Nikola, Šokarda Slavić Marinela, Vujčić Zoran, Božić Nataša, "Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases" 5 (2015),
https://doi.org/10.1038/srep15772 .

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