Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases
Апстракт
Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.
Извор:
Scientific Reports, 2015, 5Издавач:
- Nature Publishing Group, London
Финансирање / пројекти:
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-MESTD-Basic Research (BR or ON)-172048)
- ICGEB research project [CRP/YUG11-02]
DOI: 10.1038/srep15772
ISSN: 2045-2322
PubMed: 26492875
WoS: 000363430700001
Scopus: 2-s2.0-84945243760
Институција/група
IHTMTY - JOUR AU - Lončar, Nikola AU - Šokarda Slavić, Marinela AU - Vujčić, Zoran AU - Božić, Nataša PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1815 AB - Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth. PB - Nature Publishing Group, London T2 - Scientific Reports T1 - Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases VL - 5 DO - 10.1038/srep15772 ER -
@article{ author = "Lončar, Nikola and Šokarda Slavić, Marinela and Vujčić, Zoran and Božić, Nataša", year = "2015", abstract = "Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.", publisher = "Nature Publishing Group, London", journal = "Scientific Reports", title = "Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases", volume = "5", doi = "10.1038/srep15772" }
Lončar, N., Šokarda Slavić, M., Vujčić, Z.,& Božić, N.. (2015). Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases. in Scientific Reports Nature Publishing Group, London., 5. https://doi.org/10.1038/srep15772
Lončar N, Šokarda Slavić M, Vujčić Z, Božić N. Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases. in Scientific Reports. 2015;5. doi:10.1038/srep15772 .
Lončar, Nikola, Šokarda Slavić, Marinela, Vujčić, Zoran, Božić, Nataša, "Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases" in Scientific Reports, 5 (2015), https://doi.org/10.1038/srep15772 . .