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Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases

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2015
1813.pdf (514.8Kb)
Authors
Lončar, Nikola
Šokarda Slavić, Marinela
Vujčić, Zoran
Božić, Nataša
Article (Published version)
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Abstract
Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.
Source:
Scientific Reports, 2015, 5
Publisher:
  • Nature Publishing Group, London
Projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
  • ICGEB research project [CRP/YUG11-02]

DOI: 10.1038/srep15772

ISSN: 2045-2322

PubMed: 26492875

WoS: 000363430700001

Scopus: 2-s2.0-84945243760
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1815
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