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Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale

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2015
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Authors
Dojnov, Biljana
Grujic, Marica
Percevic, Bojana
Vujčić, Zoran
Article (Published version)
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Abstract
With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extract...s could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.

Keywords:
alpha-amylase / glucoamylase / maltose / starch / enzyme production / fungi
Source:
Journal of the Serbian Chemical Society, 2015, 80, 10, 1279-1288
Publisher:
  • Serbian Chemical Soc, Belgrade
Projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)

DOI: 10.2298/JSC150317043D

ISSN: 0352-5139

WoS: 000364206300005

Scopus: 2-s2.0-84957592322
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1809
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