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dc.creatorCabarkapa, A
dc.creatorBorozan, Sunčica
dc.creatorZivkovic, L
dc.creatorStojanović, Srđan
dc.creatorMilanovic-Cabarkapa, M
dc.creatorBajic, V
dc.creatorSpremo-Potparević, Biljana
dc.date.accessioned2019-01-30T17:47:14Z
dc.date.available2019-01-30T17:47:14Z
dc.date.issued2015
dc.identifier.issn0009-2797
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1800
dc.description.abstractLead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.en
dc.publisherElsevier Ireland Ltd, Clare
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.rightsrestrictedAccess
dc.sourceChemico-Biological Interactions
dc.subjectLead exposureen
dc.subjectOxidative damageen
dc.subjectGenotoxicityen
dc.subjectCaNa(2)EDTA chelationen
dc.subjectTrace elementsen
dc.titleCaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot studyen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБорозан, С; Стојановић, Срђан; Цабаркапа, A; Зивковиц, Л; Милановиц-Цабаркапа, М; Спремо-Потпаревиц, Б; Бајиц, В;
dc.citation.volume242
dc.citation.spage171
dc.citation.epage178
dc.citation.other242: 171-178
dc.citation.rankM22
dc.identifier.pmid26460059
dc.identifier.doi10.1016/j.cbi.2015.10.002
dc.identifier.rcubConv_3468
dc.identifier.scopus2-s2.0-84944406917
dc.identifier.wos000367275300018
dc.type.versionpublishedVersion


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