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dc.creatorJurica, Karlo
dc.creatorKaraconji, Irena Brcic
dc.creatorŠegan, Sandra
dc.creatorMilojković-Opsenica, Dušanka
dc.creatorKremer, Dario
dc.date.accessioned2019-01-30T17:47:04Z
dc.date.available2019-01-30T17:47:04Z
dc.date.issued2015
dc.identifier.issn0004-1254
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1792
dc.description.abstractThe phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2>0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD LT 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).en
dc.publisherInst Medical Research & Occupational Health, Zagreb
dc.relationcity of Zagreb
dc.relationMali Losinj Tourist Board
dc.rightsopenAccess
dc.sourceArhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
dc.subjectEricaceaeen
dc.subjectfood safetyen
dc.subjectGC-MSen
dc.subjectHPLCen
dc.subjectsolvent extractionen
dc.titleQuantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometryen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКарацоњи, Ирена Брциц; Шеган, Сандра; Опсеница, Дусанка Милојковиц; Кремер, Дарио; Јурица, Карло;
dc.citation.volume66
dc.citation.issue3
dc.citation.spage197
dc.citation.epage202
dc.citation.other66(3): 197-202
dc.citation.rankM23
dc.identifier.pmid26444340
dc.identifier.doi10.1515/aiht-2015-66-2696
dc.identifier.rcubConv_3459
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/8235/1790.pdf
dc.identifier.scopus2-s2.0-84944453896
dc.identifier.wos000366276100003
dc.type.versionpublishedVersion


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