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Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate

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2015
1781.pdf (901.7Kb)
Authors
Dojnov, Biljana
Grujic, Marica
Vujčić, Zoran
Article (Published version)
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Abstract
Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient ...in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.

Keywords:
alpha-amylase / glucoamylase / fungi / enzyme production / solid-state fermentation / raw starch
Source:
Journal of the Serbian Chemical Society, 2015, 80, 11, 1375-1390
Publisher:
  • Serbian Chemical Soc, Belgrade
Funding / projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)

DOI: 10.2298/JSC150317041D

ISSN: 0352-5139

WoS: 000366585800004

Scopus: 2-s2.0-84957599434
[ Google Scholar ]
8
7
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/1783
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujic, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1783
AB  - Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate
VL  - 80
IS  - 11
SP  - 1375
EP  - 1390
DO  - 10.2298/JSC150317041D
ER  - 
@article{
author = "Dojnov, Biljana and Grujic, Marica and Vujčić, Zoran",
year = "2015",
abstract = "Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate",
volume = "80",
number = "11",
pages = "1375-1390",
doi = "10.2298/JSC150317041D"
}
Dojnov, B., Grujic, M.,& Vujčić, Z.. (2015). Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(11), 1375-1390.
https://doi.org/10.2298/JSC150317041D
Dojnov B, Grujic M, Vujčić Z. Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society. 2015;80(11):1375-1390.
doi:10.2298/JSC150317041D .
Dojnov, Biljana, Grujic, Marica, Vujčić, Zoran, "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate" in Journal of the Serbian Chemical Society, 80, no. 11 (2015):1375-1390,
https://doi.org/10.2298/JSC150317041D . .

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