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dc.creatorMandić, Boris
dc.creatorVlajic, Marina D
dc.creatorTrifunović, Snežana
dc.creatorSimić, Milena R.
dc.creatorVujisić, Ljubodrag V.
dc.creatorVučković, Ivan
dc.creatorNovaković, Miroslav
dc.creatorNikolic-Mandic, Snezana D
dc.creatorTešević, Vele
dc.creatorVajs, Vlatka
dc.creatorMilosavljević, Slobodan
dc.date.accessioned2019-01-30T17:46:51Z
dc.date.available2019-01-30T17:46:51Z
dc.date.issued2015
dc.identifier.issn1478-6419
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1780
dc.description.abstractProcedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.en
dc.publisherTaylor & Francis Ltd, Abingdon
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS//
dc.rightsrestrictedAccess
dc.sourceNatural Product Research
dc.subjectisolationen
dc.subjectGC-MSen
dc.subjectpyrrolizidine alkaloidsen
dc.subjectRindera umellata Bungeen
dc.titleOptimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bungeen
dc.typearticle
dc.rights.licenseARR
dc.citation.volume29
dc.citation.issue9
dc.citation.spage887
dc.citation.epage890
dc.citation.other29(9): 887-890
dc.citation.rankM23
dc.identifier.pmid25528897
dc.identifier.doi10.1080/14786419.2014.991929
dc.identifier.rcubConv_3316
dc.identifier.scopus2-s2.0-84933044367
dc.identifier.wos000352853400019
dc.type.versionpublishedVersion


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