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dc.creatorVujčić, Zoran
dc.creatorJanović, Barbara
dc.creatorLončar, Nikola
dc.creatorMargetić, Aleksandra
dc.creatorBožić, Nataša
dc.creatorDojnov, Biljana
dc.creatorVujčić, Miroslava
dc.date.accessioned2019-01-30T17:46:24Z
dc.date.available2019-01-30T17:46:24Z
dc.date.issued2015
dc.identifier.issn0964-8305
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1760
dc.description.abstractHorseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves chromophore present.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Biodeterioration & Biodegradation
dc.subjectHorseradish peroxidaseen
dc.subjectHRPen
dc.subjectDecolorizationen
dc.subjectReactive dyesen
dc.subjectZymogramen
dc.subjectIzoenzymesen
dc.titleExploitation of neglected horseradish peroxidase izoenzymes for dye decolorizationen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујчић, Мирослава; Маргетић, Aлександра; Божић, Наташа; Дојнов, Биљана; Јановић, Барбара; Лонцар, Никола; Вујциц, Зоран;
dc.citation.volume97
dc.citation.spage124
dc.citation.epage127
dc.citation.other97: 124-127
dc.citation.rankM22
dc.identifier.doi10.1016/j.ibiod.2014.10.007
dc.identifier.rcubConv_3273
dc.identifier.scopus2-s2.0-84912567704
dc.identifier.wos000348557700016
dc.type.versionpublishedVersion


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