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dc.creatorKekez, Branka
dc.creatorGojgić-Cvijović, Gordana
dc.creatorJakovljević, Dragica
dc.creatorKojic, J R Stefanovic
dc.creatorMarković, Marijana
dc.creatorBeškoski, Vladimir
dc.creatorVrvić, Miroslav
dc.date.accessioned2019-01-30T17:46:19Z
dc.date.available2019-01-30T17:46:19Z
dc.date.issued2015
dc.identifier.issn0273-2289
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1755
dc.description.abstractIn this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm.en
dc.publisherHumana Press Inc, Totowa
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.rightsrestrictedAccess
dc.sourceApplied Biochemistry and Biotechnology
dc.subjectLevanen
dc.subjectBox-Behnken designen
dc.subjectpHen
dc.subjectBacillus licheniformisen
dc.subjectResponse surface methodologyen
dc.subjectCopperen
dc.subjectDaphnia magnaen
dc.titleHigh Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Sourceen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГојгић Цвијовић, Гордана; Јаковљевић, Драгица; Кекез, Бранка; Врвиц, М М; Бескоски, Владимир; Којиц, Ј Р Стефановиц; Марковић, Маријана;
dc.citation.volume175
dc.citation.issue6
dc.citation.spage3068
dc.citation.epage3083
dc.citation.other175(6): 3068-3083
dc.citation.rankM23
dc.identifier.pmid25592434
dc.identifier.doi10.1007/s12010-015-1475-8
dc.identifier.rcubConv_3303
dc.identifier.scopus2-s2.0-84924053585
dc.identifier.wos000350881100024
dc.type.versionpublishedVersion


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