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dc.creatorAnđelković, Uroš
dc.creatorMilutinović Nikolić, Aleksandra
dc.creatorJović-Jovičić, Nataša
dc.creatorBanković, Predrag
dc.creatorBajt, Teja
dc.creatorMojović, Zorica
dc.creatorVujčić, Zoran
dc.creatorJovanović, Dušan M.
dc.date.accessioned2019-01-30T17:45:44Z
dc.date.available2019-01-30T17:45:44Z
dc.date.issued2015
dc.identifier.issn0308-8146
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/1728
dc.description.abstractThe external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45001/RS//
dc.rightsrestrictedAccess
dc.sourceFood Chemistry
dc.subjectInvertaseen
dc.subjectImmobilisationen
dc.subjectBeidelliteen
dc.subjectSucrose hydrolysisen
dc.subjectNanoclayen
dc.titleEfficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclaysen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractAнђелковић, Урош; Бајт, Теја; Јовановиц, Дусан; Мојовић, Зорица; Банковић, Предраг; Вујциц, Зоран; Милутиновић Николић, Aлександра; Јовић-Јовичић, Наташа;
dc.citation.volume168
dc.citation.spage262
dc.citation.epage269
dc.citation.other168: 262-269
dc.citation.rankaM21
dc.identifier.pmid25172709
dc.identifier.doi10.1016/j.foodchem.2014.07.055
dc.identifier.scopus2-s2.0-84904962782
dc.identifier.wos000343612800035
dc.type.versionpublishedVersion


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