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Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex

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2015
Authors
Dojnov, Biljana
Grujic, Marica
Vujčić, Zoran
Article (Published version)
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Abstract
A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
Keywords:
beta -Glucosidase / Cellulase / Endocellulase / Exocellulase / SSF / Trichoderma / Zymogram
Source:
Electrophoresis, 2015, 36, 15, 1724-1727
Publisher:
  • Wiley-Blackwell, Hoboken
Projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
Note:
  • The peer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/3144

DOI: 10.1002/elps.201400541

ISSN: 0173-0835

PubMed: 25873475

WoS: 000359057600008

Scopus: 2-s2.0-84938380974
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1713
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