A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
TY - JOUR
AU - Dojnov, Biljana
AU - Grujic, Marica
AU - Vujčić, Zoran
PY - 2015
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/1713
AB - A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
PB - Wiley-Blackwell, Hoboken
T2 - Electrophoresis
T1 - Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex
VL - 36
IS - 15
SP - 1724
EP - 1727
DO - 10.1002/elps.201400541
ER -
@article{
author = "Dojnov, Biljana and Grujic, Marica and Vujčić, Zoran",
year = "2015",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/1713",
abstract = "A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Electrophoresis",
title = "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex",
volume = "36",
number = "15",
pages = "1724-1727",
doi = "10.1002/elps.201400541"
}
Dojnov, B., Grujic, M.,& Vujčić, Z. (2015). Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex.
Electrophoresis
Wiley-Blackwell, Hoboken., 36(15), 1724-1727.
https://doi.org/10.1002/elps.201400541
Dojnov B, Grujic M, Vujčić Z. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. Electrophoresis. 2015;36(15):1724-1727
Dojnov Biljana, Grujic Marica, Vujčić Zoran, "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex" Electrophoresis, 36, no. 15 (2015):1724-1727,
https://doi.org/10.1002/elps.201400541 .
