CER - Central Repository
Institute of Chemistry, Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Authorized Users Only
2015
Authors
Grujic, Marica
Dojnov, Biljana
Potocnik, Ivana
Duduk, Bojan
Vujčić, Zoran
Article (Published version)
Metadata
Show full item record
Abstract
Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the ...greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.

Keywords:
Spent mushroom compost / Trichoderma / Amylase / Cellulase / Xylanase
Source:
International Biodeterioration & Biodegradation, 2015, 104, 290-298
Publisher:
  • Elsevier Sci Ltd, Oxford
Funding / projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
  • Studies on plant pathogens, arthropods, weeds, and pesticides with a view to developing the methods of biorational plant protection and safe food production (RS-31043)

DOI: 10.1016/j.ibiod.2015.04.029

ISSN: 0964-8305

WoS: 000362308400041

Scopus: 2-s2.0-84934766303
[ Google Scholar ]
49
32
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/1673
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Grujic, Marica
AU  - Dojnov, Biljana
AU  - Potocnik, Ivana
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1673
AB  - Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration & Biodegradation
T1  - Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation
VL  - 104
SP  - 290
EP  - 298
DO  - 10.1016/j.ibiod.2015.04.029
ER  - 
@article{
author = "Grujic, Marica and Dojnov, Biljana and Potocnik, Ivana and Duduk, Bojan and Vujčić, Zoran",
year = "2015",
abstract = "Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration & Biodegradation",
title = "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation",
volume = "104",
pages = "290-298",
doi = "10.1016/j.ibiod.2015.04.029"
}
Grujic, M., Dojnov, B., Potocnik, I., Duduk, B.,& Vujčić, Z.. (2015). Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration & Biodegradation
Elsevier Sci Ltd, Oxford., 104, 290-298.
https://doi.org/10.1016/j.ibiod.2015.04.029
Grujic M, Dojnov B, Potocnik I, Duduk B, Vujčić Z. Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration & Biodegradation. 2015;104:290-298.
doi:10.1016/j.ibiod.2015.04.029 .
Grujic, Marica, Dojnov, Biljana, Potocnik, Ivana, Duduk, Bojan, Vujčić, Zoran, "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation" in International Biodeterioration & Biodegradation, 104 (2015):290-298,
https://doi.org/10.1016/j.ibiod.2015.04.029 . .

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB