From the aerial parts of Atriplex littoralis, three new flavonoid glycosides named atriplexins I-III (1-3), a known flavonoid glycoside, spinacetin 3-O-beta-D-glucopyranoside (4), arbutin (5), and 4-hydroxybenzyl-beta-D-glucopyranoside (6) were isolated. Their structures were elucidated on the basis of detailed spectroscopic analysis, including ID and 2D NMR (COSY, NOESY, TOCSY, HSQC, HMBC) and HRESITOF MS data. The compounds were tested for in vitro protective effects on chromosome aberrations in peripheral human lymphocytes using a cytochalasin-B-blocked micronucleus (MN) assay in a concentration range of 0.8-7.4 mu M of final culture solution. Chromosomal damage was induced by 2 Gy of gamma-radiation on binudeated human lymphocytes, and the effects of the compounds were tested 2 to 19 h after irradiation. The frequency of micronuclei (MNi) was scored in binudeated cells, and the nuclear proliferation index was calculated. The highest prevention of in vitro biochemical and cytogeneti...c damage of human lymphocytes induced by gamma-radiation was exhibited by 3 (reduction of MN frequency by 31%), followed by 4 and 6.
Извор:
Journal of Natural Products, 2015, 78, 9, 2198-2204
TY - JOUR
AU - Gođevac, Dejan
AU - Stanković, Jovana
AU - Novaković, Miroslav
AU - Anđelković, Boban D.
AU - Dajić-Stevanović, Zora
AU - Petrovic, Milica
AU - Stanković, Miroslava
PY - 2015
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1672
AB - From the aerial parts of Atriplex littoralis, three new flavonoid glycosides named atriplexins I-III (1-3), a known flavonoid glycoside, spinacetin 3-O-beta-D-glucopyranoside (4), arbutin (5), and 4-hydroxybenzyl-beta-D-glucopyranoside (6) were isolated. Their structures were elucidated on the basis of detailed spectroscopic analysis, including ID and 2D NMR (COSY, NOESY, TOCSY, HSQC, HMBC) and HRESITOF MS data. The compounds were tested for in vitro protective effects on chromosome aberrations in peripheral human lymphocytes using a cytochalasin-B-blocked micronucleus (MN) assay in a concentration range of 0.8-7.4 mu M of final culture solution. Chromosomal damage was induced by 2 Gy of gamma-radiation on binudeated human lymphocytes, and the effects of the compounds were tested 2 to 19 h after irradiation. The frequency of micronuclei (MNi) was scored in binudeated cells, and the nuclear proliferation index was calculated. The highest prevention of in vitro biochemical and cytogenetic damage of human lymphocytes induced by gamma-radiation was exhibited by 3 (reduction of MN frequency by 31%), followed by 4 and 6.
PB - American Chemical Society (ACS)
T2 - Journal of Natural Products
T1 - Phenolic Compounds from Atriplex littoralis and Their Radiation-Mitigating Activity
VL - 78
IS - 9
SP - 2198
EP - 2204
DO - 10.1021/acs.jnatprod.5b00273
ER -
@article{
author = "Gođevac, Dejan and Stanković, Jovana and Novaković, Miroslav and Anđelković, Boban D. and Dajić-Stevanović, Zora and Petrovic, Milica and Stanković, Miroslava",
year = "2015",
abstract = "From the aerial parts of Atriplex littoralis, three new flavonoid glycosides named atriplexins I-III (1-3), a known flavonoid glycoside, spinacetin 3-O-beta-D-glucopyranoside (4), arbutin (5), and 4-hydroxybenzyl-beta-D-glucopyranoside (6) were isolated. Their structures were elucidated on the basis of detailed spectroscopic analysis, including ID and 2D NMR (COSY, NOESY, TOCSY, HSQC, HMBC) and HRESITOF MS data. The compounds were tested for in vitro protective effects on chromosome aberrations in peripheral human lymphocytes using a cytochalasin-B-blocked micronucleus (MN) assay in a concentration range of 0.8-7.4 mu M of final culture solution. Chromosomal damage was induced by 2 Gy of gamma-radiation on binudeated human lymphocytes, and the effects of the compounds were tested 2 to 19 h after irradiation. The frequency of micronuclei (MNi) was scored in binudeated cells, and the nuclear proliferation index was calculated. The highest prevention of in vitro biochemical and cytogenetic damage of human lymphocytes induced by gamma-radiation was exhibited by 3 (reduction of MN frequency by 31%), followed by 4 and 6.",
publisher = "American Chemical Society (ACS)",
journal = "Journal of Natural Products",
title = "Phenolic Compounds from Atriplex littoralis and Their Radiation-Mitigating Activity",
volume = "78",
number = "9",
pages = "2198-2204",
doi = "10.1021/acs.jnatprod.5b00273"
}
Gođevac, D., Stanković, J., Novaković, M., Anđelković, B. D., Dajić-Stevanović, Z., Petrovic, M.,& Stanković, M.. (2015). Phenolic Compounds from Atriplex littoralis and Their Radiation-Mitigating Activity. in Journal of Natural Products
American Chemical Society (ACS)., 78(9), 2198-2204.
https://doi.org/10.1021/acs.jnatprod.5b00273
Gođevac D, Stanković J, Novaković M, Anđelković BD, Dajić-Stevanović Z, Petrovic M, Stanković M. Phenolic Compounds from Atriplex littoralis and Their Radiation-Mitigating Activity. in Journal of Natural Products. 2015;78(9):2198-2204.
doi:10.1021/acs.jnatprod.5b00273 .
Gođevac, Dejan, Stanković, Jovana, Novaković, Miroslav, Anđelković, Boban D., Dajić-Stevanović, Zora, Petrovic, Milica, Stanković, Miroslava, "Phenolic Compounds from Atriplex littoralis and Their Radiation-Mitigating Activity" in Journal of Natural Products, 78, no. 9 (2015):2198-2204,
https://doi.org/10.1021/acs.jnatprod.5b00273 . .
