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dc.creatorTrendafilova, Antoaneta
dc.creatorJadranin, Milka
dc.creatorGorgorov, Rossen
dc.creatorStanilova, Marina
dc.date.accessioned2019-01-30T17:44:11Z
dc.date.available2019-01-30T17:44:11Z
dc.date.issued2015
dc.identifier.issn1934-578X
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1653
dc.description.abstractIn vitro cultures were initiated from a single seed of Centaurea davidovii. Whole plantlets were regenerated and cultivated for several months on agar-solidified nutrient media differing by their composition: basal MS medium, MS medium supplemented with plant growth regulators, and liquid MS medium. Plantlets were ex vitro adapted and successfully acclimated to open-air conditions; flowering was observed in some individuals in the first summer, and mass flowering during the second summer. The contents of the total flavonoids and the total phenolic compounds were determined spectrophotometrically in the leaves of the in vitro plantlets cultured on different media, and then compared with those in the leaves of the wild plants and in the leaves of the acclimated plants of the field plot. The sesquiterpene lactone 8 alpha-(5'-hydroxyangeloyl)-salonitenolide was determined by HPLC in leaf samples of C. davidovii wild plants, in vitro obtained plantlets and ex vitro acclimated plants in the greenhouse and on the experimental field plot. The composition of the nutrient medium influenced the contents of all studied bioactive substances. The highest concentrations of all tested secondary metabolites were detected in the leaves of the acclimated plants during mass flowering, the content of the lactone reaching 56.2 mg/g DW, which was several times more than in the other leaf samples. The obtained results revealed both the effectiveness of biotechnological methods for propagation and conservation of rare and endangered plant species, and the possibility to use C. davidovii plants ex vitro acclimated to field conditions as a source of secondary metabolites with potential biological activity.en
dc.publisherNatural Products Inc, Westerville
dc.rightsrestrictedAccess
dc.sourceNatural Product Communications
dc.subjectCentaureaen
dc.subjectin vitro Culturesen
dc.subjectSesquiterpene lactonesen
dc.subjectFlavonoidsen
dc.subjectPolyphenolsen
dc.titleBioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae)en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈадранин, Милка; Трендафилова, Aнтоанета; Горгоров, Россен; Станилова, Марина;
dc.citation.volume10
dc.citation.issue6
dc.citation.spage839
dc.citation.epage841
dc.citation.other10(6): 839-841
dc.citation.rankM23
dc.identifier.pmid26197494
dc.identifier.rcubConv_3354
dc.identifier.wos000356565700007
dc.type.versionpublishedVersion


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