Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.
TY - JOUR
AU - Tosti, Tomislav
AU - Šegan, Sandra
AU - Milić, Dragana
AU - Radoičić, Aleksandra D.
AU - Tešić, Živoslav
AU - Milojković-Opsenica, Dušanka
PY - 2015
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/1646
AB - Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.
PB - Taylor & Francis Inc, Philadelphia
T2 - Journal of Liquid Chromatography & Related Technologies
T1 - Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography
VL - 38
IS - 11
SP - 1097
EP - 1103
DO - 10.1080/10826076.2015.1028287
ER -
@article{
author = "Tosti, Tomislav and Šegan, Sandra and Milić, Dragana and Radoičić, Aleksandra D. and Tešić, Živoslav and Milojković-Opsenica, Dušanka",
year = "2015",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/1646",
abstract = "Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography",
volume = "38",
number = "11",
pages = "1097-1103",
doi = "10.1080/10826076.2015.1028287"
}
Tosti T, Šegan S, Milić D, Radoičić AD, Tešić Ž, Milojković-Opsenica D. Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography. Journal of Liquid Chromatography & Related Technologies. 2015;38(11):1097-1103
Tosti, T., Šegan, S., Milić, D., Radoičić, A. D., Tešić, Ž.,& Milojković-Opsenica, D. (2015). Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography.
Journal of Liquid Chromatography & Related TechnologiesTaylor & Francis Inc, Philadelphia., 38(11), 1097-1103.
https://doi.org/10.1080/10826076.2015.1028287
Tosti Tomislav, Šegan Sandra, Milić Dragana, Radoičić Aleksandra D., Tešić Živoslav, Milojković-Opsenica Dušanka, "Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography" 38, no. 11 (2015):1097-1103,
https://doi.org/10.1080/10826076.2015.1028287 .
