Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit
Само за регистроване кориснике
2014
Аутори
Mrkić, Ivan
Abughren, Mohamed
Nikolić, Jasna

Anđelković, Uroš

Vassilopoulou, Emilia
Sinaniotis, Athanassios
Petersen, Arnd
Papadopoulos, Nikolaos G.

Gavrović-Jankulović, Marija

Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition t...est. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.
Кључне речи:
Allergen / Banana glucanase / Food allergy / IgE reactivity / Mus a 5Извор:
Molecular Biotechnology, 2014, 56, 6, 498-506Издавач:
- Humana Press Inc, Totowa
Финансирање / пројекти:
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-172049)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
DOI: 10.1007/s12033-013-9719-8
ISSN: 1073-6085
PubMed: 24198217
WoS: 000335504400002
Scopus: 2-s2.0-84900792894
Институција/група
IHTMTY - JOUR AU - Mrkić, Ivan AU - Abughren, Mohamed AU - Nikolić, Jasna AU - Anđelković, Uroš AU - Vassilopoulou, Emilia AU - Sinaniotis, Athanassios AU - Petersen, Arnd AU - Papadopoulos, Nikolaos G. AU - Gavrović-Jankulović, Marija PY - 2014 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1579 AB - Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy. PB - Humana Press Inc, Totowa T2 - Molecular Biotechnology T1 - Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit VL - 56 IS - 6 SP - 498 EP - 506 DO - 10.1007/s12033-013-9719-8 ER -
@article{ author = "Mrkić, Ivan and Abughren, Mohamed and Nikolić, Jasna and Anđelković, Uroš and Vassilopoulou, Emilia and Sinaniotis, Athanassios and Petersen, Arnd and Papadopoulos, Nikolaos G. and Gavrović-Jankulović, Marija", year = "2014", abstract = "Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.", publisher = "Humana Press Inc, Totowa", journal = "Molecular Biotechnology", title = "Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit", volume = "56", number = "6", pages = "498-506", doi = "10.1007/s12033-013-9719-8" }
Mrkić, I., Abughren, M., Nikolić, J., Anđelković, U., Vassilopoulou, E., Sinaniotis, A., Petersen, A., Papadopoulos, N. G.,& Gavrović-Jankulović, M.. (2014). Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit. in Molecular Biotechnology Humana Press Inc, Totowa., 56(6), 498-506. https://doi.org/10.1007/s12033-013-9719-8
Mrkić I, Abughren M, Nikolić J, Anđelković U, Vassilopoulou E, Sinaniotis A, Petersen A, Papadopoulos NG, Gavrović-Jankulović M. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit. in Molecular Biotechnology. 2014;56(6):498-506. doi:10.1007/s12033-013-9719-8 .
Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, "Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit" in Molecular Biotechnology, 56, no. 6 (2014):498-506, https://doi.org/10.1007/s12033-013-9719-8 . .