Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit
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2014
Authors
Mrkić, Ivan
Abughren, Mohamed
Nikolić, Jasna

Anđelković, Uroš

Vassilopoulou, Emilia
Sinaniotis, Athanassios
Petersen, Arnd
Papadopoulos, Nikolaos G.

Gavrović-Jankulović, Marija

Article (Published version)

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Show full item recordAbstract
Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition t...est. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.
Keywords:
Allergen / Banana glucanase / Food allergy / IgE reactivity / Mus a 5Source:
Molecular Biotechnology, 2014, 56, 6, 498-506Publisher:
- Humana Press Inc, Totowa
Projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
DOI: 10.1007/s12033-013-9719-8
ISSN: 1073-6085
PubMed: 24198217