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Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells

Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša

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2004
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Authors
Radojičić, Ratko M.
Spasić, Snežana
Saičić, Zorica S.
Jovanović, Tamara B.
Simić-Krstić, Jovana B.
Contribution To Periodical (Published version)
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Abstract
The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.
C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.
Keywords:
B-16 mouse melanoma / isoenzymes / α-naphthyl acetate esterase (ANAE) / leucine aminopeptidase (LAP) / copper / zinc–containing superoxide dismutase (CuZn SOD)
Source:
Journal of the Serbian Chemical Society, 2004, 69, 12, 1005-1011
Publisher:
  • Serbian Chemical Society
Projects:
  • Project MESTD, br. 1669

DOI: 10.2298/JSC0412005R

ISSN: 0352-5139

WoS: 000226998500003

Scopus: 2-s2.0-31544434923
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/155
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