CER - Central Repository
Institute of Chemistry, Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells

Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša

Thumbnail
2004
153.pdf (163.5Kb)
Authors
Radojičić, Ratko M.
Spasić, Snežana
Saičić, Zorica S.
Jovanović, Tamara B.
Simić-Krstić, Jovana B.
Contribution To Periodical (Published version)
Metadata
Show full item record
Abstract
The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.
C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.
Keywords:
B-16 mouse melanoma / isoenzymes / α-naphthyl acetate esterase (ANAE) / leucine aminopeptidase (LAP) / copper / zinc–containing superoxide dismutase (CuZn SOD)
Source:
Journal of the Serbian Chemical Society, 2004, 69, 12, 1005-1011
Publisher:
  • Serbian Chemical Society
Funding / projects:
  • Project MESTD, br. 1669

DOI: 10.2298/JSC0412005R

ISSN: 0352-5139

WoS: 000226998500003

Scopus: 2-s2.0-31544434923
[ Google Scholar ]
1
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/155
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Radojičić, Ratko M.
AU  - Spasić, Snežana
AU  - Saičić, Zorica S.
AU  - Jovanović, Tamara B.
AU  - Simić-Krstić, Jovana B.
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/155
AB  - The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.
AB  - C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells
T1  - Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša
VL  - 69
IS  - 12
SP  - 1005
EP  - 1011
DO  - 10.2298/JSC0412005R
ER  - 
@article{
author = "Radojičić, Ratko M. and Spasić, Snežana and Saičić, Zorica S. and Jovanović, Tamara B. and Simić-Krstić, Jovana B.",
year = "2004",
abstract = "The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation., C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells, Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša",
volume = "69",
number = "12",
pages = "1005-1011",
doi = "10.2298/JSC0412005R"
}
Radojičić, R. M., Spasić, S., Saičić, Z. S., Jovanović, T. B.,& Simić-Krstić, J. B.. (2004). Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 69(12), 1005-1011.
https://doi.org/10.2298/JSC0412005R
Radojičić RM, Spasić S, Saičić ZS, Jovanović TB, Simić-Krstić JB. Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells. in Journal of the Serbian Chemical Society. 2004;69(12):1005-1011.
doi:10.2298/JSC0412005R .
Radojičić, Ratko M., Spasić, Snežana, Saičić, Zorica S., Jovanović, Tamara B., Simić-Krstić, Jovana B., "Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells" in Journal of the Serbian Chemical Society, 69, no. 12 (2004):1005-1011,
https://doi.org/10.2298/JSC0412005R . .

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB