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dc.creatorMilicevic, Zorka
dc.creatorBajic, Vladan
dc.creatorŽivković, Lada
dc.creatorKasapovic, Jelena
dc.creatorAnđelković, Uroš
dc.creatorSpremo-Potparević, Biljana
dc.date.accessioned2019-01-30T17:41:41Z
dc.date.available2019-01-30T17:41:41Z
dc.date.issued2014
dc.identifier.issn1537-744X
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1535
dc.description.abstractIn breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.en
dc.publisherHindawi Publishing Corporation, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.rightsopenAccess
dc.sourceScientific World Journal
dc.titleIdentification of p53 and Its Isoforms in Human Breast Carcinoma Cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКасаповиц, Јелена; Зивковиц, Лада; Спремо-Потпаревиц, Биљана; Бајиц, Владан; Aнђелковић, Урош; Милицевиц, Зорка;
dc.citation.other
dc.citation.rankM21
dc.identifier.pmid24511294
dc.identifier.doi10.1155/2014/618698
dc.identifier.rcubrcub_1533
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/13576/1533.pdf
dc.identifier.scopus2-s2.0-84893205816
dc.identifier.wos000330388500001
dc.type.versionpublishedVersion


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