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Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells

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2014
1533.pdf (2.621Mb)
Authors
Milicevic, Zorka
Bajic, Vladan
Živković, Lada
Kasapovic, Jelena
Anđelković, Uroš
Spremo-Potparević, Biljana
Article (Published version)
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Abstract
In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) ...and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.

Source:
Scientific World Journal, 2014
Publisher:
  • Hindawi Publishing Corporation, New York
Projects:
  • Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-173034)

DOI: 10.1155/2014/618698

ISSN: 1537-744X

PubMed: 24511294

WoS: 000330388500001

Scopus: 2-s2.0-84893205816
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1535
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