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dc.creatorProkopovic, Vladimir
dc.creatorPopović, Milica
dc.creatorAnđelković, Uroš
dc.creatorMarsavelski, Aleksandra
dc.creatorRaskovic, Brankica
dc.creatorGavrović-Jankulović, Marija
dc.creatorPolović, Natalija
dc.date.accessioned2019-01-30T17:41:04Z
dc.date.available2019-01-30T17:41:04Z
dc.date.issued2014
dc.identifier.issn0003-9969
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1508
dc.description.abstractObjective: Human BPIFA2 (parotid secretory protein) is a ubiquitous soluble salivary protein, which belongs to the PLUNC family of proteins. Having sequence similarity to bactericidal/permeability-increasing protein and lipopolysaccharide-binding protein, PLUNC proteins are probably involved in local antibacterial response at mucosal sites, such as oral cavity. The aim of the study was to isolate and characterize human BPIFA2. Design: In this paper, we report one-step affinity chromatography method for BPIFA2 purification from whole human saliva. The isolated BPIFA2 was identified by trypsin mass fingerprinting and characterized by electrophoretic methods. Antibacterial activity of BPIFA2 against model microorganism Pseudomonas aeruginosa was shown in minimum inhibitory concentration and time kill study assays. Results: The protein showed microheterogeneity, both in molecular weight and pI value. BPIFA2 inhibited the growth of P. aeruginosa in microgram concentration range determined by minimum inhibitory concentration assay. In the time kill study, 32 mu g/mL BPIFA2 showed clear bactericidal activity and did not cause any aggregation of bacteria. Conclusion: Affinity chromatography is well suited for isolation of functional BPIFA2 with a potent bactericidal activity against P. aeruginosa.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceArchives of Oral Biology
dc.subjectParotid secretory proteinen
dc.subjectBPIFA2en
dc.subjectSalivaen
dc.subjectBactericidal activityen
dc.titleIsolation, biochemical characterization and anti-bacterial activity of BPIFA2 proteinen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПоловиц, Наталија; Поповиц, Милица; Aнђелковић, Урош; Расковиц, Бранкица; Прокоповиц, Владимир; Гавровиц-Јанкуловиц, Марија; Марсавелски, Aлександра;
dc.citation.volume59
dc.citation.issue3
dc.citation.spage302
dc.citation.epage309
dc.citation.other59(3): 302-309
dc.citation.rankM21
dc.identifier.pmid24581853
dc.identifier.doi10.1016/j.archoralbio.2013.12.005
dc.identifier.rcubConv_3132
dc.identifier.scopus2-s2.0-84891760970
dc.identifier.wos000333489600010
dc.type.versionpublishedVersion


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