Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases
Апстракт
Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.
Кључне речи:
Bacillus sp. / alpha-amylase / isoform / raw starch hydrolysisИзвор:
Archives of Biological Sciences, 2014, 66, 2, 483-490Издавач:
- Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Финансирање / пројекти:
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-MESTD-Basic Research (BR or ON)-172048)
- ICGEB [CRP/YUG11-02]
DOI: 10.2298/ABS1402483S
ISSN: 0354-4664
WoS: 000342265800005
Scopus: 2-s2.0-84904012889
Институција/група
IHTMTY - JOUR AU - Šokarda Slavić, Marinela AU - Božić, Nataša AU - Vujčić, Zoran PY - 2014 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1439 AB - Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis. PB - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd T2 - Archives of Biological Sciences T1 - Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases VL - 66 IS - 2 SP - 483 EP - 490 DO - 10.2298/ABS1402483S ER -
@article{ author = "Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran", year = "2014", abstract = "Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.", publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd", journal = "Archives of Biological Sciences", title = "Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases", volume = "66", number = "2", pages = "483-490", doi = "10.2298/ABS1402483S" }
Šokarda Slavić, M., Božić, N.,& Vujčić, Z.. (2014). Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases. in Archives of Biological Sciences Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 66(2), 483-490. https://doi.org/10.2298/ABS1402483S
Šokarda Slavić M, Božić N, Vujčić Z. Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases. in Archives of Biological Sciences. 2014;66(2):483-490. doi:10.2298/ABS1402483S .
Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, "Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases" in Archives of Biological Sciences, 66, no. 2 (2014):483-490, https://doi.org/10.2298/ABS1402483S . .