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Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases

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2014
1437.pdf (633.1Kb)
Authors
Šokarda Slavić, Marinela
Božić, Nataša
Vujčić, Zoran
Article (Published version)
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Abstract
Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.
Keywords:
Bacillus sp. / alpha-amylase / isoform / raw starch hydrolysis
Source:
Archives of Biological Sciences, 2014, 66, 2, 483-490
Publisher:
  • Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Funding / projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
  • ICGEB [CRP/YUG11-02]

DOI: 10.2298/ABS1402483S

ISSN: 0354-4664

WoS: 000342265800005

Scopus: 2-s2.0-84904012889
[ Google Scholar ]
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/1439
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1439
AB  - Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of Biological Sciences
T1  - Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases
VL  - 66
IS  - 2
SP  - 483
EP  - 490
DO  - 10.2298/ABS1402483S
UR  - Conv_3222
ER  - 
@article{
author = "Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran",
year = "2014",
abstract = "Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of Biological Sciences",
title = "Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases",
volume = "66",
number = "2",
pages = "483-490",
doi = "10.2298/ABS1402483S",
url = "Conv_3222"
}
Šokarda Slavić, M., Božić, N.,& Vujčić, Z.. (2014). Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases. in Archives of Biological Sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 66(2), 483-490.
https://doi.org/10.2298/ABS1402483S
Conv_3222
Šokarda Slavić M, Božić N, Vujčić Z. Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases. in Archives of Biological Sciences. 2014;66(2):483-490.
doi:10.2298/ABS1402483S
Conv_3222 .
Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, "Growth temperature of different local isolates of bacillus sp in the solid state affects production of raw starch digesting amylases" in Archives of Biological Sciences, 66, no. 2 (2014):483-490,
https://doi.org/10.2298/ABS1402483S .,
Conv_3222 .

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