Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation
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2014
Authors
Bajcetic, MilicaOtasevic, Biljana

Prekajski, Niveska Bozinovic
Spasić, Snežana

Spasojević, Ivan

Article (Published version)

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Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared... to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.
Keywords:
Enzymes / enzymatic methods / spectrometrySource:
Annals of Clinical Biochemistry, 2014, 51, 5, 550-556Publisher:
- Sage Publications Inc, Thousand Oaks
Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-173014)
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
DOI: 10.1177/0004563213503317
ISSN: 0004-5632
PubMed: 24081186
WoS: 000344251100004
Scopus: 2-s2.0-84906330485
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Institution/Community
IHTMTY - JOUR AU - Bajcetic, Milica AU - Otasevic, Biljana AU - Prekajski, Niveska Bozinovic AU - Spasić, Snežana AU - Spasojević, Ivan PY - 2014 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1416 AB - Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational. PB - Sage Publications Inc, Thousand Oaks T2 - Annals of Clinical Biochemistry T1 - Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation VL - 51 IS - 5 SP - 550 EP - 556 DO - 10.1177/0004563213503317 ER -
@article{ author = "Bajcetic, Milica and Otasevic, Biljana and Prekajski, Niveska Bozinovic and Spasić, Snežana and Spasojević, Ivan", year = "2014", abstract = "Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.", publisher = "Sage Publications Inc, Thousand Oaks", journal = "Annals of Clinical Biochemistry", title = "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation", volume = "51", number = "5", pages = "550-556", doi = "10.1177/0004563213503317" }
Bajcetic, M., Otasevic, B., Prekajski, N. B., Spasić, S.,& Spasojević, I.. (2014). Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry Sage Publications Inc, Thousand Oaks., 51(5), 550-556. https://doi.org/10.1177/0004563213503317
Bajcetic M, Otasevic B, Prekajski NB, Spasić S, Spasojević I. Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry. 2014;51(5):550-556. doi:10.1177/0004563213503317 .
Bajcetic, Milica, Otasevic, Biljana, Prekajski, Niveska Bozinovic, Spasić, Snežana, Spasojević, Ivan, "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation" in Annals of Clinical Biochemistry, 51, no. 5 (2014):550-556, https://doi.org/10.1177/0004563213503317 . .