Effect of methyl oleate and Tween 80 on the antibiotic productivity and the fatty acid composition of the total lipids of Streptomyces hygroscopicus CH-7

Abstract

The effects of methyl oleate and Tween 80, hexaene H-85 production stimulators, on the fatty acid composition of the total lipids of S. hygroscopicus CH-7 were investigated. Besides oleic acid in the biomass, an increase of the branched chain fatty acid contents was observed: from 1.6 to 2.1 fold in the medium with methyl oleate and 2.5-2.9 in the medium with Tween 80 compared to the base medium. These two additives act differently on the growth of the producing strain and the uptake of valine, an essential nutrient. A decreased proteolytic activity and similar cellular fatty acid profiles indicate that the main effect of the added compounds is to facilitate the excretion of antibiotics to the outer medium, thus increasing biosynthesis.

U radu je ispitivano dejstvo u podlogu dodatih metil oleata i Tween-a 80, stimulatora produkcije heksaena N-85, na sadržaj masnih kiselina u ukupnim lipidima soja S. hygroscopicus CH-7. Pored povećanja sadržaja oleinske kiseline u biomasi pri fermentaciji na podlozi sa metil oleatom sadržaj i 16:0, 17:0, 15:0 i ai 17:0, 15:0 je od 1,6 do 2,1 puta viši, dok na podlozi sa Tween-om 80 ovo povećanje iznosi 2,5-2,9 puta. Ova dva aditiva različito deluju na rast produkcionog soja kao i na brzinu usvajanja valina iz podloge. Smanjenje proteolitičke aktivnosti i sličnosti u sastavu lipida ukazuju da je verovatno najvažnije dejstvo metil oleata i Tween-a 80 stimulacija ekskrecije polienskog antibiotika u podlogu čime se povećava njegova sinteza.