Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification
Само за регистроване кориснике
2014
Аутори
Kozarski, Maja S.
Klaus, Anita

Jakovljević, Dragica

Todorović, Nina

Nikšić, Miomir

Vrvić, Miroslav

Van Griensven, Leo J. L. D.
Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O...2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.
Кључне речи:
A. brasiliensis / Polysaccharide extracts / Immunomodulation / Antioxidant activity / EPR spin trappingИзвор:
Food Research International, 2014, 64, 53-64Издавач:
- Elsevier
Финансирање / пројекти:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
- Развој и примена нових и традиционалних технологија у производњи конкурентних прехрамбених производа са додатом вредношћу за европско и светско тржиште - Створимо богатство из богатства Србије (RS-46001)
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-46010)
- Симултана биоремедијација и соилификација деградираних простора, за очување природних ресурса биолошки активних супстанци и развој и производњу биоматеријала и дијететских производа (RS-43004)
DOI: 10.1016/j.foodres.2014.05.075
ISSN: 0963-9969
PubMed: 30011685
WoS: 000343840700007
Scopus: 2-s2.0-84903193477
Институција/група
IHTMTY - JOUR AU - Kozarski, Maja S. AU - Klaus, Anita AU - Jakovljević, Dragica AU - Todorović, Nina AU - Nikšić, Miomir AU - Vrvić, Miroslav AU - Van Griensven, Leo J. L. D. PY - 2014 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1395 AB - Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement. PB - Elsevier T2 - Food Research International T1 - Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification VL - 64 SP - 53 EP - 64 DO - 10.1016/j.foodres.2014.05.075 ER -
@article{ author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.", year = "2014", abstract = "Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.", publisher = "Elsevier", journal = "Food Research International", title = "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification", volume = "64", pages = "53-64", doi = "10.1016/j.foodres.2014.05.075" }
Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2014). Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International Elsevier., 64, 53-64. https://doi.org/10.1016/j.foodres.2014.05.075
Kozarski MS, Klaus A, Jakovljević D, Todorović N, Nikšić M, Vrvić M, Van Griensven LJLD. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International. 2014;64:53-64. doi:10.1016/j.foodres.2014.05.075 .
Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification" in Food Research International, 64 (2014):53-64, https://doi.org/10.1016/j.foodres.2014.05.075 . .