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Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification

Authorized Users Only
2014
Authors
Kozarski, Maja S.
Klaus, Anita
Jakovljević, Dragica
Todorović, Nina
Nikšić, Miomir
Vrvić, Miroslav
Van Griensven, Leo J. L. D.
Article (Published version)
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Abstract
Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O...2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.

Keywords:
A. brasiliensis / Polysaccharide extracts / Immunomodulation / Antioxidant activity / EPR spin trapping
Source:
Food Research International, 2014, 64, 53-64
Publisher:
  • Elsevier
Funding / projects:
  • Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
  • Develooment and utilization of novel and traditional technologies in production of competitive food products with added valued for national and global market - CREATING WEALTH FROM THE WEALTH OF SERBIA (RS-46001)
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)

DOI: 10.1016/j.foodres.2014.05.075

ISSN: 0963-9969

PubMed: 30011685

WoS: 000343840700007

Scopus: 2-s2.0-84903193477
[ Google Scholar ]
23
19
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/1395
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1395
AB  - Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.
PB  - Elsevier
T2  - Food Research International
T1  - Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification
VL  - 64
SP  - 53
EP  - 64
DO  - 10.1016/j.foodres.2014.05.075
ER  - 
@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2014",
abstract = "Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification",
volume = "64",
pages = "53-64",
doi = "10.1016/j.foodres.2014.05.075"
}
Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2014). Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International
Elsevier., 64, 53-64.
https://doi.org/10.1016/j.foodres.2014.05.075
Kozarski MS, Klaus A, Jakovljević D, Todorović N, Nikšić M, Vrvić M, Van Griensven LJLD. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International. 2014;64:53-64.
doi:10.1016/j.foodres.2014.05.075 .
Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification" in Food Research International, 64 (2014):53-64,
https://doi.org/10.1016/j.foodres.2014.05.075 . .

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