Show simple item record

dc.creatorDojnov, Biljana
dc.creatorPavlovic, Ratko
dc.creatorBožić, Nataša
dc.creatorMargetić, Aleksandra
dc.creatorNenadovic, Vera
dc.creatorIvanovic, Jelisaveta
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:38:10Z
dc.date.available2019-01-30T17:38:10Z
dc.date.issued2013
dc.identifier.issn1096-4959
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1366
dc.description.abstractThe influence of diet composition - two substrates, wheat bran and sawdust - on isoform expression of digestive enzymes (cellulase, amylase and peptidase) in the midgut of Morimus funereus larvae was examined. Their impact on larval development was demonstrated by measuring the increase of larval weight during development and by analysis of digestive enzymes zymographic profiles, where the expression of cellulase isoforms from M. funereus larvae midgut has been examined for the first time in this study. Larvae reared on wheat bran had higher body weight between day 60 and day 100 than larvae reared on sawdust; however, both groups achieved similar body weight after day 110. Wheat bran as substrate induced different cellulase and amylase isoforms. Oak sawdust in substrate acted as inducer of peptidases. The highest cellulase activity and the greatest isoform variability were detected in the midgut extracts of larvae reared on wheat bran. From our results it can be assumed that M. funereus endocellulase, amylase and peptidase are secreted in the anterior midgut, and their concentration gradually decreases towards the hindguten
dc.publisherElsevier Science Inc, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsrestrictedAccess
dc.sourceComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
dc.subjectAmylaseen
dc.subjectCellulaseen
dc.subjectIsoformsen
dc.subjectMorimus funereusen
dc.subjectPeptidaseen
dc.titleExpression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate compositionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБожић, Наташа; Ивановиц, Јелисавета; Павловиц, Ратко; Дојнов, Биљана; Маргетић, Aлександра; Ненадовиц, Вера; Вујциц, Зоран;
dc.citation.volume164
dc.citation.issue4
dc.citation.spage259
dc.citation.epage267
dc.citation.other164(4): 259-267
dc.citation.rankM21
dc.identifier.pmid23402787
dc.identifier.doi10.1016/j.cbpb.2013.02.001
dc.identifier.rcubConv_2955
dc.identifier.scopus2-s2.0-84874703557
dc.identifier.wos000317325400004
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record