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Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition

Samo za registrovane korisnike
2013
Autori
Dojnov, Biljana
Pavlovic, Ratko
Božić, Nataša
Margetić, Aleksandra
Nenadovic, Vera
Ivanovic, Jelisaveta
Vujčić, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
The influence of diet composition - two substrates, wheat bran and sawdust - on isoform expression of digestive enzymes (cellulase, amylase and peptidase) in the midgut of Morimus funereus larvae was examined. Their impact on larval development was demonstrated by measuring the increase of larval weight during development and by analysis of digestive enzymes zymographic profiles, where the expression of cellulase isoforms from M. funereus larvae midgut has been examined for the first time in this study. Larvae reared on wheat bran had higher body weight between day 60 and day 100 than larvae reared on sawdust; however, both groups achieved similar body weight after day 110. Wheat bran as substrate induced different cellulase and amylase isoforms. Oak sawdust in substrate acted as inducer of peptidases. The highest cellulase activity and the greatest isoform variability were detected in the midgut extracts of larvae reared on wheat bran. From our results it can be assumed that M. funere...us endocellulase, amylase and peptidase are secreted in the anterior midgut, and their concentration gradually decreases towards the hindgut

Ključne reči:
Amylase / Cellulase / Isoforms / Morimus funereus / Peptidase
Izvor:
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 2013, 164, 4, 259-267
Izdavač:
  • Elsevier Science Inc, New York
Projekti:
  • Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-172048)

DOI: 10.1016/j.cbpb.2013.02.001

ISSN: 1096-4959

PubMed: 23402787

WoS: 000317325400004

Scopus: 2-s2.0-84874703557
[ Google Scholar ]
4
4
URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1366
Kolekcije
  • Radovi istraživača / Researchers' publications
Institucija
IHTM
TY  - JOUR
AU  - Dojnov, Biljana
AU  - Pavlovic, Ratko
AU  - Božić, Nataša
AU  - Margetić, Aleksandra
AU  - Nenadovic, Vera
AU  - Ivanovic, Jelisaveta
AU  - Vujčić, Zoran
PY  - 2013
UR  - http://cer.ihtm.bg.ac.rs/handle/123456789/1366
AB  - The influence of diet composition - two substrates, wheat bran and sawdust - on isoform expression of digestive enzymes (cellulase, amylase and peptidase) in the midgut of Morimus funereus larvae was examined. Their impact on larval development was demonstrated by measuring the increase of larval weight during development and by analysis of digestive enzymes zymographic profiles, where the expression of cellulase isoforms from M. funereus larvae midgut has been examined for the first time in this study. Larvae reared on wheat bran had higher body weight between day 60 and day 100 than larvae reared on sawdust; however, both groups achieved similar body weight after day 110. Wheat bran as substrate induced different cellulase and amylase isoforms. Oak sawdust in substrate acted as inducer of peptidases. The highest cellulase activity and the greatest isoform variability were detected in the midgut extracts of larvae reared on wheat bran. From our results it can be assumed that M. funereus endocellulase, amylase and peptidase are secreted in the anterior midgut, and their concentration gradually decreases towards the hindgut
PB  - Elsevier Science Inc, New York
T2  - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
T1  - Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition
VL  - 164
IS  - 4
SP  - 259
EP  - 267
DO  - 10.1016/j.cbpb.2013.02.001
ER  - 
@article{
author = "Dojnov, Biljana and Pavlovic, Ratko and Božić, Nataša and Margetić, Aleksandra and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran",
year = "2013",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/1366",
abstract = "The influence of diet composition - two substrates, wheat bran and sawdust - on isoform expression of digestive enzymes (cellulase, amylase and peptidase) in the midgut of Morimus funereus larvae was examined. Their impact on larval development was demonstrated by measuring the increase of larval weight during development and by analysis of digestive enzymes zymographic profiles, where the expression of cellulase isoforms from M. funereus larvae midgut has been examined for the first time in this study. Larvae reared on wheat bran had higher body weight between day 60 and day 100 than larvae reared on sawdust; however, both groups achieved similar body weight after day 110. Wheat bran as substrate induced different cellulase and amylase isoforms. Oak sawdust in substrate acted as inducer of peptidases. The highest cellulase activity and the greatest isoform variability were detected in the midgut extracts of larvae reared on wheat bran. From our results it can be assumed that M. funereus endocellulase, amylase and peptidase are secreted in the anterior midgut, and their concentration gradually decreases towards the hindgut",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",
title = "Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition",
volume = "164",
number = "4",
pages = "259-267",
doi = "10.1016/j.cbpb.2013.02.001"
}
Dojnov B, Pavlovic R, Božić N, Margetić A, Nenadovic V, Ivanovic J, Vujčić Z. Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2013;164(4):259-267
Dojnov, B., Pavlovic, R., Božić, N., Margetić, A., Nenadovic, V., Ivanovic, J.,& Vujčić, Z. (2013). Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition.
Comparative Biochemistry and Physiology - B Biochemistry and Molecular BiologyElsevier Science Inc, New York., 164(4), 259-267.
https://doi.org/10.1016/j.cbpb.2013.02.001
Dojnov Biljana, Pavlovic Ratko, Božić Nataša, Margetić Aleksandra, Nenadovic Vera, Ivanovic Jelisaveta, Vujčić Zoran, "Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L. (Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition" 164, no. 4 (2013):259-267,
https://doi.org/10.1016/j.cbpb.2013.02.001 .

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