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Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays

Authorized Users Only
2013
Authors
Gorjanović, Stanislava Ž.
Alvarez-Suarez, Jose Miguel
Novaković, Miroslav
Pastor, Ferenc T.
Pezo, Lato
Battino, Maurizio
Suznjevic, Desanka Z.
Article (Published version)
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Abstract
Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxid...ant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.

Keywords:
Analytical method / Food analysis / Honey / Honey constituents / Food composition / Phenolics / Reducing sugars / Antioxidant activity / Hydrogen peroxide scavenge / DC polarography / Anodic current / Honey adul
Source:
Journal of Food Composition and Analysis, 2013, 30, 1, 13-18
Publisher:
  • Academic Press Inc Elsevier Science, San Diego
Funding / projects:
  • Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-43010)
  • Content of bioactive components in small and stone fruits as affected by cultivar specificities and growing conditions, and obtaining biologically valuable products by improved and newly developed technologies (RS-31093)

DOI: 10.1016/j.jfca.2012.12.004

ISSN: 0889-1575

WoS: 000317799500003

Scopus: 2-s2.0-84873531795
[ Google Scholar ]
80
72
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/1357
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Gorjanović, Stanislava Ž.
AU  - Alvarez-Suarez, Jose Miguel
AU  - Novaković, Miroslav
AU  - Pastor, Ferenc T.
AU  - Pezo, Lato
AU  - Battino, Maurizio
AU  - Suznjevic, Desanka Z.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1357
AB  - Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays
VL  - 30
IS  - 1
SP  - 13
EP  - 18
DO  - 10.1016/j.jfca.2012.12.004
ER  - 
@article{
author = "Gorjanović, Stanislava Ž. and Alvarez-Suarez, Jose Miguel and Novaković, Miroslav and Pastor, Ferenc T. and Pezo, Lato and Battino, Maurizio and Suznjevic, Desanka Z.",
year = "2013",
abstract = "Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays",
volume = "30",
number = "1",
pages = "13-18",
doi = "10.1016/j.jfca.2012.12.004"
}
Gorjanović, S. Ž., Alvarez-Suarez, J. M., Novaković, M., Pastor, F. T., Pezo, L., Battino, M.,& Suznjevic, D. Z.. (2013). Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 30(1), 13-18.
https://doi.org/10.1016/j.jfca.2012.12.004
Gorjanović SŽ, Alvarez-Suarez JM, Novaković M, Pastor FT, Pezo L, Battino M, Suznjevic DZ. Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. in Journal of Food Composition and Analysis. 2013;30(1):13-18.
doi:10.1016/j.jfca.2012.12.004 .
Gorjanović, Stanislava Ž., Alvarez-Suarez, Jose Miguel, Novaković, Miroslav, Pastor, Ferenc T., Pezo, Lato, Battino, Maurizio, Suznjevic, Desanka Z., "Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays" in Journal of Food Composition and Analysis, 30, no. 1 (2013):13-18,
https://doi.org/10.1016/j.jfca.2012.12.004 . .

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