The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have... an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.
TY - JOUR
AU - Miladinovic, Dragoljub L.
AU - Ilic, Budimir S.
AU - Najman, Stevo J.
AU - Cvetković, Olga
AU - Šajnović, Aleksandra
AU - Miladinovic, Marija D.
AU - Nikolic, Nikola D.
PY - 2013
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/1320
AB - The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.
PB - Versita, Warsaw
T2 - Central European Journal of Chemistry
T1 - Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract
VL - 11
IS - 2
SP - 123
EP - 132
DO - 10.2478/s11532-012-0163-6
ER -
@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Najman, Stevo J. and Cvetković, Olga and Šajnović, Aleksandra and Miladinovic, Marija D. and Nikolic, Nikola D.",
year = "2013",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/1320",
abstract = "The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Chemistry",
title = "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract",
volume = "11",
number = "2",
pages = "123-132",
doi = "10.2478/s11532-012-0163-6"
}
Miladinovic, D. L., Ilic, B. S., Najman, S. J., Cvetković, O., Šajnović, A., Miladinovic, M. D.,& Nikolic, N. D. (2013). Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract.
Central European Journal of Chemistry
Versita, Warsaw., 11(2), 123-132.
https://doi.org/10.2478/s11532-012-0163-6
Miladinovic DL, Ilic BS, Najman SJ, Cvetković O, Šajnović A, Miladinovic MD, Nikolic ND. Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. Central European Journal of Chemistry. 2013;11(2):123-132
Miladinovic Dragoljub L., Ilic Budimir S., Najman Stevo J., Cvetković Olga, Šajnović Aleksandra, Miladinovic Marija D., Nikolic Nikola D., "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract" Central European Journal of Chemistry, 11, no. 2 (2013):123-132,
https://doi.org/10.2478/s11532-012-0163-6 .
