Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
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2013
Authors
Prlainović, Nevena Ž.
Bezbradica, Dejan I.
Knežević-Jugović, Zorica

Stevanović, Sanja

Avramov Ivić, Milka

Uskoković, Petar S.

Mijin, Dušan

Article (Published version)

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Show full item recordAbstract
In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
Keywords:
Lipase / Carbon nanotubes / Adsorption / FT-IR spectroscopy / AFM / Cyclic voltammetrySource:
Journal of Industrial and Engineering Chemistry, 2013, 19, 1, 279-285Publisher:
- Korean Society of Industrial Engineering Chemistry
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Synthesis, processing and applications of nanostructured multifunctional materials with defined properties (RS-45019)
DOI: 10.1016/j.jiec.2012.08.012
ISSN: 1226-086X
WoS: 000312764700039
Scopus: 2-s2.0-84870399209
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IHTMTY - JOUR AU - Prlainović, Nevena Ž. AU - Bezbradica, Dejan I. AU - Knežević-Jugović, Zorica AU - Stevanović, Sanja AU - Avramov Ivić, Milka AU - Uskoković, Petar S. AU - Mijin, Dušan PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1297 AB - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry. PB - Korean Society of Industrial Engineering Chemistry T2 - Journal of Industrial and Engineering Chemistry T1 - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes VL - 19 IS - 1 SP - 279 EP - 285 DO - 10.1016/j.jiec.2012.08.012 ER -
@article{ author = "Prlainović, Nevena Ž. and Bezbradica, Dejan I. and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov Ivić, Milka and Uskoković, Petar S. and Mijin, Dušan", year = "2013", abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.", publisher = "Korean Society of Industrial Engineering Chemistry", journal = "Journal of Industrial and Engineering Chemistry", title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes", volume = "19", number = "1", pages = "279-285", doi = "10.1016/j.jiec.2012.08.012" }
Prlainović, N. Ž., Bezbradica, D. I., Knežević-Jugović, Z., Stevanović, S., Avramov Ivić, M., Uskoković, P. S.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry Korean Society of Industrial Engineering Chemistry., 19(1), 279-285. https://doi.org/10.1016/j.jiec.2012.08.012
Prlainović NŽ, Bezbradica DI, Knežević-Jugović Z, Stevanović S, Avramov Ivić M, Uskoković PS, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285. doi:10.1016/j.jiec.2012.08.012 .
Prlainović, Nevena Ž., Bezbradica, Dejan I., Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov Ivić, Milka, Uskoković, Petar S., Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285, https://doi.org/10.1016/j.jiec.2012.08.012 . .