Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors
Abstract
We analyze the fluctuations of the equilibrium adsorbed mass in affinity-based biosensors, considering adsorption-desorption and mass transfer processes of two molecular species which compete for binding to the same probe molecules immobilized on the sensing surface. The analytical expression for the fluctuations spectral density is derived. The calculations show the significant influence of both the competitive adsorption and the mass transfer on the fluctuations spectrum, and also of their combined effect. The dependences of the fluctuations on association and dissociation rate constants of both adsorbing species, their concentrations, molecular masses and mass transfer coefficients and also on the density of capturing probes can be examined by using the presented model. The model is applicable for biosensors in which the spatial distribution of adsorbates concentrations in the reaction chamber can be approximated using the two-compartment model. The theory provides a more realistic ...estimation of the biosensors' limiting performance, and is useful for their improvement.
Keywords:
Biosensor / Competitive binding / Adsorption-desorption / Mass transfer / FluctuationsSource:
Sensors and Actuators, B: Chemical, 2013, 189, 71-79Publisher:
- Elsevier
Funding / projects:
- Micro- Nanosystems and Sensors for Electric Power and Process Industry and Environmental Protection (RS-32008)
- Serbian Academy of Sciences and Arts, Project F-150
DOI: 10.1016/j.snb.2012.12.080
ISSN: 0925-4005
WoS: 000326346300011
Scopus: 2-s2.0-84887061320
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Institution/Community
IHTMTY - JOUR AU - Frantlović, Miloš AU - Jokić, Ivana AU - Đurić, Zoran G. AU - Radulović, Katarina PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1255 AB - We analyze the fluctuations of the equilibrium adsorbed mass in affinity-based biosensors, considering adsorption-desorption and mass transfer processes of two molecular species which compete for binding to the same probe molecules immobilized on the sensing surface. The analytical expression for the fluctuations spectral density is derived. The calculations show the significant influence of both the competitive adsorption and the mass transfer on the fluctuations spectrum, and also of their combined effect. The dependences of the fluctuations on association and dissociation rate constants of both adsorbing species, their concentrations, molecular masses and mass transfer coefficients and also on the density of capturing probes can be examined by using the presented model. The model is applicable for biosensors in which the spatial distribution of adsorbates concentrations in the reaction chamber can be approximated using the two-compartment model. The theory provides a more realistic estimation of the biosensors' limiting performance, and is useful for their improvement. PB - Elsevier T2 - Sensors and Actuators, B: Chemical T1 - Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors VL - 189 SP - 71 EP - 79 DO - 10.1016/j.snb.2012.12.080 ER -
@article{ author = "Frantlović, Miloš and Jokić, Ivana and Đurić, Zoran G. and Radulović, Katarina", year = "2013", abstract = "We analyze the fluctuations of the equilibrium adsorbed mass in affinity-based biosensors, considering adsorption-desorption and mass transfer processes of two molecular species which compete for binding to the same probe molecules immobilized on the sensing surface. The analytical expression for the fluctuations spectral density is derived. The calculations show the significant influence of both the competitive adsorption and the mass transfer on the fluctuations spectrum, and also of their combined effect. The dependences of the fluctuations on association and dissociation rate constants of both adsorbing species, their concentrations, molecular masses and mass transfer coefficients and also on the density of capturing probes can be examined by using the presented model. The model is applicable for biosensors in which the spatial distribution of adsorbates concentrations in the reaction chamber can be approximated using the two-compartment model. The theory provides a more realistic estimation of the biosensors' limiting performance, and is useful for their improvement.", publisher = "Elsevier", journal = "Sensors and Actuators, B: Chemical", title = "Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors", volume = "189", pages = "71-79", doi = "10.1016/j.snb.2012.12.080" }
Frantlović, M., Jokić, I., Đurić, Z. G.,& Radulović, K.. (2013). Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors. in Sensors and Actuators, B: Chemical Elsevier., 189, 71-79. https://doi.org/10.1016/j.snb.2012.12.080
Frantlović M, Jokić I, Đurić ZG, Radulović K. Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors. in Sensors and Actuators, B: Chemical. 2013;189:71-79. doi:10.1016/j.snb.2012.12.080 .
Frantlović, Miloš, Jokić, Ivana, Đurić, Zoran G., Radulović, Katarina, "Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors" in Sensors and Actuators, B: Chemical, 189 (2013):71-79, https://doi.org/10.1016/j.snb.2012.12.080 . .