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dc.creatorVujčić, Miroslava
dc.creatorTufegdžić, Srđan
dc.creatorNovaković, Irena
dc.creatorDjikanovic, Daniela
dc.creatorGašić, Miroslav J.
dc.creatorSladić, Dušan
dc.date.accessioned2019-01-30T17:35:24Z
dc.date.available2019-01-30T17:35:24Z
dc.date.issued2013
dc.identifier.issn0141-8130
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/1237
dc.description.abstractThe interactions of avarone, a quinone from the marine sponge Dysideaavara, and the methylamino derivatives of avarone (2), 3'-(methylamino)avarone (3) and 4'-(methylamino)avarone (4) with calf thymus DNA (CT-DNA) were studied. Agarose gel electrophoreticanalysis showed that binding of the quinones quenched fluorescence of ethidium bromide (EB). The extent of fluorescence quenching of intercalator EB by competitive displacement from EB-CT-DNA system and of groove binder Hoechst 33258 (H) from H-CT-DNA system with the quinones was analyzed by fluorescence spectroscopy. The obtained results demonstrated that the quinones reduced binding of both the intercalator EB and the minor groove binder H, indicating possible degradation of DNA. The substituent on the quinone moiety determined the extent of DNA damaging effect of the quinone, which was the most extensive with 3'-(methylamino)avarone and the least extensive with its regioisomer 4'-(methylamino)avarone. The results were confirmed by the observed hyperchromic effects in UV-visible spectra measured after interactions of the derivatives with CT-DNA.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172055/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectAvarolen
dc.subjectAvaroneen
dc.subject3 '-(Methylamino)avaroneen
dc.subject4 '-(Methylamino)avaroneen
dc.subjectCalf thymus DNAen
dc.subjectFluorescence measurementsen
dc.titleStudies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNAen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДјикановиц, Даниела; Гасиц, Мирослав Ј.; Новаковић, Ирена; Сладиц, Дусан; Туфегджић, Срђан; Вујчић, Мирослава;
dc.citation.volume62
dc.citation.spage405
dc.citation.epage410
dc.citation.other62: 405-410
dc.citation.rankM22
dc.identifier.pmid24080453
dc.identifier.doi10.1016/j.ijbiomac.2013.09.013
dc.identifier.rcubConv_3097
dc.identifier.scopus2-s2.0-84886041638
dc.identifier.wos000329881200060
dc.type.versionpublishedVersion


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