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Antioxidant activity of propolis extracts from Serbia: A polarographic approach
dc.creator | Potkonjak, Nebojsa I. | |
dc.creator | Veselinović, Dragan S. | |
dc.creator | Novaković, Miroslav | |
dc.creator | Gorjanović, Stanislava Ž. | |
dc.creator | Pezo, Lato | |
dc.creator | Suznjevic, Desanka Z. | |
dc.date.accessioned | 2019-01-30T17:33:08Z | |
dc.date.available | 2019-01-30T17:33:08Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0278-6915 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/1128 | |
dc.description.abstract | Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. | en |
dc.publisher | Oxford : Pergamon-Elsevier Science Ltd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172015/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43010/RS// | |
dc.rights | restrictedAccess | |
dc.source | Food and Chemical Toxicology | |
dc.subject | Propolis extract | en |
dc.subject | Antioxidant activity | en |
dc.subject | Hydrogen peroxide | en |
dc.subject | Polarography | en |
dc.title | Antioxidant activity of propolis extracts from Serbia: A polarographic approach | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Горјановиц, Станислава З.; Поткоњак, Небојса И.; Новаковић, Мирослав; Сузњевиц, Десанка З.; Веселиновиц, Драган С.; Пезо, Лато Л.; | |
dc.citation.volume | 50 | |
dc.citation.issue | 10 | |
dc.citation.spage | 3614 | |
dc.citation.epage | 3618 | |
dc.citation.other | 50(10): 3614-3618 | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 22842121 | |
dc.identifier.doi | 10.1016/j.fct.2012.07.029 | |
dc.identifier.scopus | 2-s2.0-84865518003 | |
dc.identifier.wos | 000309897100032 | |
dc.type.version | publishedVersion |