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Molecular and immunological characterization of Mus a 5 allergen from banana fruit

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2012
Authors
Aleksić, Ivana
Popović, Milica
Dimitrijević, Rajna
Anđelković, Uroš
Vassilopoulou, Emilia
Sinaniotis, Athanassios
Atanasković-Marković, Marina
Lindner, Buko
Petersen, Arnd
Papadopoulos, Nikolaos G.
Gavrović-Jankulović, Marija
Article (Published version)
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Abstract
Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensiti...zed to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Keywords:
Allergen / Banana / Food allergy / Glucanase / Mus a 5
Source:
Molecular Nutrition & Food Research, 2012, 56, 3, 446-453
Publisher:
  • Wiley-Blackwell, Malden
Projects:
  • Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)

DOI: 10.1002/mnfr.201100541

ISSN: 1613-4125

PubMed: 22162266

WoS: 000302169000008

Scopus: 2-s2.0-84859181170
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/1024
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