Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives
Apstrakt
A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
Izvor:
Bioconjugate Chemistry, 2012, 23, 1, 57-65Izdavač:
- American Chemical Society (ACS)
Finansiranje / projekti:
- Interakcije prirodnih proizvoda, njihovih derivata i kompleksnih jedinjenja sa proteinima i nukleinskim kiselinama (RS-172055)
- Deutscher Akademischer Austausch Dienst (DAAD)
Napomena:
- This is the peer-reviewed version of the article: Novaković, I., Anđelković, U., Zlatović, M., Gašić, M.J., Sladić, D., 2012. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. Bioconjugate Chem. 23, 57–65. https://doi.org/10.1021/bc200330m
DOI: 10.1021/bc200330m
ISSN: 1043-1802
PubMed: 22148500
WoS: 000299591000007
Scopus: 2-s2.0-84855919661
Institucija/grupa
IHTMTY - JOUR AU - Novaković, Irena AU - Anđelković, Uroš AU - Zlatović, Mario AU - Gašić, Miroslav J. AU - Sladić, Dušan PY - 2012 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1005 AB - A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates. PB - American Chemical Society (ACS) T2 - Bioconjugate Chemistry T1 - Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives VL - 23 IS - 1 SP - 57 EP - 65 DO - 10.1021/bc200330m ER -
@article{ author = "Novaković, Irena and Anđelković, Uroš and Zlatović, Mario and Gašić, Miroslav J. and Sladić, Dušan", year = "2012", abstract = "A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.", publisher = "American Chemical Society (ACS)", journal = "Bioconjugate Chemistry", title = "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives", volume = "23", number = "1", pages = "57-65", doi = "10.1021/bc200330m" }
Novaković, I., Anđelković, U., Zlatović, M., Gašić, M. J.,& Sladić, D.. (2012). Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry American Chemical Society (ACS)., 23(1), 57-65. https://doi.org/10.1021/bc200330m
Novaković I, Anđelković U, Zlatović M, Gašić MJ, Sladić D. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry. 2012;23(1):57-65. doi:10.1021/bc200330m .
Novaković, Irena, Anđelković, Uroš, Zlatović, Mario, Gašić, Miroslav J., Sladić, Dušan, "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives" in Bioconjugate Chemistry, 23, no. 1 (2012):57-65, https://doi.org/10.1021/bc200330m . .